公开(公告)号：US10022728B2

公开(公告)日：2018-07-17

申请号：US14764875

申请日：2014-02-03

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Mark Hayes ,  Paul Jones ,  Stacy Kenyon ,  Michael Keebaugh ,  Thomas Taylor ,  Prasun Mahanti ,  Sarah Staton ,  Noah Weiss

IPC: G01N27/447 ,  G01N27/453 ,  B03C7/02 ,  B03C5/00 ,  G01N30/00 ,  B03C5/02 ,  B01L3/00 ,  G06F17/50

Abstract: The present invention provides devices and methods to separate and concentrate target species. In some embodiments, a punctuated continuous microchannel or parallel processing (array-based) separations are provided, the microchannel having a plurality of sequential, constrictive insulating features to form a plurality of reservoirs including a first, second and third reservoir, and a plurality of constricted passageways including a first constricted passageway that connects the first reservoir to the second reservoir and a second constricted passageway that connects the second reservoir to the third reservoir. A voltage is applied to the microchannel to create different electrical fields and/or different dielectrophoresis (DEP) gradients at each of the plurality of constricted passageways in order to separate species that have differing ratios of electrokinetic mobility to dielectrophoretic mobility.