公开(公告)号：US20220064719A1

公开(公告)日：2022-03-03

申请号：US17461816

申请日：2021-08-30

Applicant: Applied Materials, Inc.

Inventor： Chloe Kim ,  Lu Yan

IPC: C12Q1/6841 ,  G01N21/64 ,  G06T7/80

Abstract: A method of calibrating a fluorescence in-situ hybridization (FISH) system includes contacting a calibration slide with a plurality of probes, obtaining one or more images of the calibration slide; and calibrating the FISH system based on the one or more images. The calibration slide has a surface with a first plurality of beads. Each bead of the first plurality of beads has one or more binding domains. Each probe has a tag and a targeting domain, and the targeting domain binds a binding domain from the at least one binding domain.

公开(公告)号：US20220066190A1

公开(公告)日：2022-03-03

申请号：US17461827

申请日：2021-08-30

Applicant: Applied Materials, Inc.

Inventor： Chloe Kim ,  Lu Yan

IPC: G02B21/34 ,  G01N21/64

Abstract: A calibration slide for calibrating a multiplexed fluorescence in-situ hybridization (FISH) system has a substrate having a surface and a first plurality of beads on the surface. Each bead of the first plurality of beads has at least one binding domain to be bound to a corresponding probe that has a tag and a targeting domain that binds to a binding domain.

公开(公告)号：US20220065787A1

公开(公告)日：2022-03-03

申请号：US17460052

申请日：2021-08-27

Applicant: Applied Materials, Inc.

Inventor： Chloe Kim ,  Lu Yan

IPC: G01N21/64 ,  C12Q1/6837 ,  C12Q1/6874

Abstract: This disclosure relates to methods for image registration in fluorescence assays for the detection and quantitation of analytes in a sample using functionalized alignment beads as fiducial markers to improve image registration.

公开(公告)号：US20220064718A1

公开(公告)日：2022-03-03

申请号：US17460056

申请日：2021-08-27

Applicant: Applied Materials, Inc.

Inventor： Chloe Kim ,  Lu Yan

IPC: C12Q1/6837 ,  C12Q1/6818

Abstract: Functionalized alignment beads each have a plurality of binding sites, the plurality binding sites including a first subset and a second subset of binding sites. The first subset of binding sites include a first nucleotide sequence selected to bind to a complementary first nucleotide sequence of a first probe that has a fluorophore and that targets the first nucleotide sequence in a sample or in a first targeting probe that targets the sample. The second subset of the plurality of binding sites include a second nucleotide sequence selected to bind to a complementary second nucleotide sequence of a second probe that has a fluorophore and that targets the second nucleotide sequence in a sample or in a second targeting probe that targets the sample. The fluorophore of the first probe has a same emission wavelength and/or same excitation wavelength as the fluorophore of the second probe.