公开(公告)号：US20240344114A1

公开(公告)日：2024-10-17

申请号：US18744391

申请日：2024-06-14

申请人： Grifols Diagnostic Solutions Inc.

发明人： Uffe Vest Schneider ,  Søren Morgenthaler Echwald ,  Nikolaj Dam Mikkelsen

IPC分类号： C12Q1/6818

CPC分类号： C12Q1/6818

摘要： The present invention relates to a method for detecting at least one target nucleic acid sequence from a nucleic acid mixture by a double quenched assay. The double quenched assay of the method exploits a novel approach for melting temperature mediated identification of multiple target nucleic acid sequences. The invention further relates to a kit of parts.

公开(公告)号：US20240336957A1

公开(公告)日：2024-10-10

申请号：US18635474

申请日：2024-04-15

申请人： California Institute of Technology ,  Molecular Instruments

发明人： Harry MT Choi ,  Chun Hao R. Chen ,  Mike C. Liu ,  Aneesh Acharya ,  Sheldon Zhu ,  Niles A. Pierce

IPC分类号： C12Q1/682 ,  C12Q1/6818 ,  C12Q1/6841

CPC分类号： C12Q1/682 ,  C12Q1/6818 ,  C12Q1/6841

摘要： The present application relates to hybridization chain reaction (HCR). In particular, compositions and methods are presented for ultrasensitive molecular detection using HCR signal amplification. Some embodiments and methods involve cooperative probe junctions, reporter-labeled probes, and nonlinear HCR signal amplification.

公开(公告)号：US20240309430A1

公开(公告)日：2024-09-19

申请号：US18608081

申请日：2024-03-18

申请人： The Hong Kong University of Science and Technology

发明人： I-Ming HSING ,  Xinyu ZHUANG

IPC分类号： C12Q1/6818 ,  C12Q1/6844

CPC分类号： C12Q1/6818 ,  C12Q1/6844

摘要： The subject invention pertains to the multiplex detection of nucleic acid molecules. This invention provides oligonucleotides labeled with a fluorophore and a quencher and oligonucleotides with target binding regions that are broadly compatible with the detection of multiple variant locus or multiple nucleic acid samples. Additionally, this disclosure also describes methods to label detection targets by asymmetric labeled amplification and differentiate the target sequences.

公开(公告)号：US12091706B2

公开(公告)日：2024-09-17

申请号：US16965368

申请日：2019-01-29

申请人： Rush University Medical Center

发明人： Vineet Gupta ,  Anugraha Rajagopalan

IPC分类号： C12Q1/68 ,  C07H21/00 ,  C12Q1/6806 ,  C12Q1/6818

CPC分类号： C12Q1/6818 ,  C07H21/00 ,  C12Q1/6806

摘要： Compositions and methods are provided including a plurality of analyte detection agents, each analyte detection agent includes a labile tag operatively coupled to the analyte detection agent, each labile tag includes a signal that is different from each other labile tag and each analyte detection agent targeting a different analyte.

公开(公告)号：US20240294969A1

公开(公告)日：2024-09-05

申请号：US18492337

申请日：2023-10-23

申请人： Nanogami GmbH

发明人： Jean-Philippe Jacques Sobczak ,  Sumersing Virendrasing Patil ,  Tamara Bernadette Aigner-Bogner ,  Ali Aghebat Rafat

IPC分类号： C12Q1/6818

CPC分类号： C12Q1/6818 ,  B82Y40/00

摘要： The disclosure relates to systems and methods for binding payload moieties to a surface. In particular, the binding is mediated by nucleic acid nanostructures.

公开(公告)号：US20240279754A1

公开(公告)日：2024-08-22

申请号：US18443665

申请日：2024-02-16

申请人： UNIVERSITY OF MARYLAND, BALTIMORE ,  ISS Inc.

发明人： Krishanu RAY ,  Anthony L. DEVICO ,  Beniamino BARBIERI ,  Yuansheng SUN

IPC分类号： C12Q1/70 ,  C12Q1/02 ,  C12Q1/6818 ,  G01N21/64 ,  G16B40/10 ,  G16H20/17

CPC分类号： C12Q1/703 ,  C12Q1/025 ,  C12Q1/6818 ,  G01N21/6486 ,  G16B40/10 ,  G16H20/17

摘要： A fluorescence-based analytical platform comprising methods, software and instrumentation that accomplishes direct, rapid, high throughput, multiplex, and quantitative determinations of virion populations, in human or animals or experimental fluids, that are impacted by, or escape from, interactions with antiviral drugs. The platform can be used to advance bnAb resistance detection capacities to support numerous bnAb clinical trial activities, from screening volunteers to tailoring subject-specific bnAb combinations for treatment or cure.

公开(公告)号：US12024737B2

公开(公告)日：2024-07-02

申请号：US17396148

申请日：2021-08-06

申请人： Bio-Rad Laboratories, Inc.

发明人： John F. Regan ,  Dianna Maar ,  Dawne N. Shelton ,  Tina C. Legler ,  Samantha Cooper ,  Wei Yang ,  Eli A. Hefner ,  Niels Klitgord

IPC分类号： C12Q1/68 ,  C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6848

CPC分类号： C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6848 ,  C12Q1/6818 ,  C12Q2527/107 ,  C12Q2565/1015 ,  C12Q1/6823 ,  C12Q2537/1373 ,  C12Q2545/107 ,  C12Q2565/101

摘要： Method of analysis for alleles of a target. In the method, a plurality of fluid volumes may be formed. Each fluid volume may contain a first primer pair to amplify a first allele of a target, a second primer pair to amplify a second allele of the target, a first reporter including a first photoluminophore and providing a primer of the first primer pair, and a second reporter including a second photoluminophore and providing a primer of the second primer pair. Each of the first and second reporters may include an oligomer having a quencher, and the oligomer may be configured to base-pair with the primer of the first primer pair and the primer of the second primer pair. The first and second alleles may be amplified using the first and second primer pairs. Photoluminescence may be detected. A level of each allele may be determined.

公开(公告)号：US20240209462A1

公开(公告)日：2024-06-27

申请号：US18577860

申请日：2022-07-12

申请人： Nanyang Technological University ,  Agency for Science, Technology and Research

发明人： Meng How TAN ,  Kean Hean OOI

IPC分类号： C12Q1/70 ,  C12Q1/6818 ,  C12Q1/6844

CPC分类号： C12Q1/701 ,  C12Q1/6818 ,  C12Q1/6844

摘要： Various embodiments relate generally to the field of nucleic acid amplification and detection, in particular isothermal nucleic acid amplification and the detection of the amplicons using designated detection probes. Moreover, various embodiments also relate to methods for determining the presence or quantity of a target nucleic acid molecule in a sample using isothermal amplification. The detection probe is a single-stranded probe that recognises a probe binding site within target amplicons. The detection probe comprises at least one 3′end nucleotide mismatch and a quencher-fluorophore pair at the opposite ends of the probe. Following hybridization of the detection probe to the target amplicons, a DNA polymerase with 3′-5′ exonuclease activity can cleave the detection probe at the 3′ end nucleotide mismatch to release a 3′-terminal probe fragment comprising the quencher or fluorophore, thus generating signals.

公开(公告)号：US20240209426A1

公开(公告)日：2024-06-27

申请号：US18540659

申请日：2023-12-14

申请人： NAT DIAGNOSTICS, INC.

发明人： Andrew P. Miller ,  Honghua Zhang

IPC分类号： C12Q1/6844 ,  C12Q1/6816 ,  C12Q1/6818 ,  C12Q1/686 ,  C12Q1/689 ,  G01N21/64

CPC分类号： C12Q1/6844 ,  C12Q1/6816 ,  C12Q1/6818 ,  C12Q1/686 ,  C12Q1/689 ,  G01N21/6428 ,  C12Q2521/101 ,  C12Q2521/107 ,  C12Q2527/101 ,  C12Q2527/113 ,  C12Q2531/113 ,  C12Q2561/113 ,  C12Q2600/16 ,  G01N2021/6432

摘要： The technology relates in part to methods and compositions for isothermal amplification of nucleic acids.

公开(公告)号：US12018322B2

公开(公告)日：2024-06-25

申请号：US17646819

申请日：2022-01-03

申请人： Mobiusloop BioScience Limited

发明人： Chung Wai Jonathan Lam ,  Chi Hang Wong ,  Chi Hang Wong

IPC分类号： C12Q1/6844 ,  C12Q1/00 ,  C12Q1/6818

CPC分类号： C12Q1/6844 ,  C12Q1/004 ,  C12Q1/6818

摘要： Provided herein are methods involving an autocatalyzed looped relay amplification mechanism for detecting an analyte in a sample. The method is useful for detecting very low abundance analytes.