公开(公告)号：US10947576B2

公开(公告)日：2021-03-16

申请号：US16633812

申请日：2018-07-25

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Nongjian Tao ,  Karan Syal

IPC: G06K9/00 ,  C12Q1/18 ,  G01N15/14 ,  G01N33/543 ,  G01N15/00

Abstract: A method for rapid antibiotic susceptibility testing by tracking sub-micron scale motion of single bacterial cells including obtaining a biological sample from a subject including live bacteria. Different doses of antibiotic are added to a multi-well glass slide and adding portions of the biological sample to the wells. Bacterial cells are tethered onto the surface. The tethered bacterial cells are imaged and tracked. Bacterial sub-micron motion of tethered cells is measured at the different doses. A processor performs statistical analysis on a population of cells for each antibiotic dose to generate an antibiotic dose curve proportional to the motion changes, where the antibiotic dose curve plots data including a decrease in movement over time indicating a proportional effectiveness of an antibiotic applied to a well.

公开(公告)号：US20200172951A1

公开(公告)日：2020-06-04

申请号：US16633812

申请日：2018-07-25

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Nongjian Tao ,  Karan Syal

IPC: C12Q1/18 ,  G01N33/543 ,  G01N15/14 ,  G06K9/00

Abstract: A method for rapid antibiotic susceptibility testing by tracking sub-micron scale motion of single bacterial cells including obtaining a biological sample from a subject including live bacteria. Different doses of antibiotic are added to a multi-well glass slide and adding portions of the biological sample to the wells. Bacterial cells are tethered onto the surface. The tethered bacterial cells are imaged and tracked. Bacterial sub-micron motion of tethered cells is measured at the different doses. A processor performs statistical analysis on a population of cells for each antibiotic dose to generate an antibiotic dose curve proportional to the motion changes, where the antibiotic dose curve plots data including a decrease in movement over time indicating a proportional effectiveness of an antibiotic applied to a well.

公开(公告)号：US20210054432A1

公开(公告)日：2021-02-25

申请号：US17072742

申请日：2020-10-16

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Nongjian Tao ,  Karan Syal

IPC: C12Q1/18 ,  G01N15/14 ,  G01N33/543 ,  G06K9/00

Abstract: A method for rapid antibiotic susceptibility testing by tracking sub-micron scale motion of single bacterial cells including obtaining a biological sample from a subject including live bacteria. Different doses of antibiotic are added to a multi-well glass slide and adding portions of the biological sample to the wells. Bacterial cells are tethered onto the surface. The tethered bacterial cells are imaged and tracked. Bacterial sub-micron motion of tethered cells is measured at the different doses. A processor performs statistical analysis on a population of cells for each antibiotic dose to generate an antibiotic dose curve proportional to the motion changes, where the antibiotic dose curve plots data including a decrease in movement over time indicating a proportional effectiveness of an antibiotic applied to a well.

公开(公告)号：US11198897B2

公开(公告)日：2021-12-14

申请号：US17072742

申请日：2020-10-16

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Nongjian Tao ,  Karan Syal

IPC: G06K9/00 ,  C12Q1/18 ,  G01N15/14 ,  G01N33/543 ,  G01N15/00

Abstract: A method for rapid antibiotic susceptibility testing by tracking sub-micron scale motion of single bacterial cells including obtaining a biological sample from a subject including live bacteria. Different doses of antibiotic are added to a multi-well glass slide and adding portions of the biological sample to the wells. Bacterial cells are tethered onto the surface. The tethered bacterial cells are imaged and tracked. Bacterial sub-micron motion of tethered cells is measured at the different doses. A processor performs statistical analysis on a population of cells for each antibiotic dose to generate an antibiotic dose curve proportional to the motion changes, where the antibiotic dose curve plots data including a decrease in movement over time indicating a proportional effectiveness of an antibiotic applied to a well.