公开(公告)号：US20200095564A1

公开(公告)日：2020-03-26

申请号：US16700322

申请日：2019-12-02

Applicant: BASF SE

Inventor： Christian Degering ,  Thorsten Eggert ,  Stefan Evers ,  Karl-Heinz Maure ,  Johannes Bongaerts

IPC: C12N9/50 ,  C12N15/75 ,  C12N15/70 ,  C12N15/62

Abstract: The aim of the invention is to improve the secretion of a protein from a host cell in order to increase the product yield of protein in a fermentation process. This is achieved by an expression vector comprising a) a promoter sequence and b) a nucleic acid sequence that codes for a protein. The protein comprises a signal peptide and an additional amino acid sequence, and the signal peptide comprises an amino acid sequence that is at least 80% identical to the amino acid sequence specified in SEQ ID NO: 2, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 4, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 6, or the signal peptide comprises an amino acid sequence that is structurally homologous to at least one of said sequences.