公开(公告)号：US07972994B2

公开(公告)日：2011-07-05

申请号：US11450938

申请日：2006-06-09

申请人： Barry Morgan ,  Stephen Hale ,  Christopher C. Arico-Muendel ,  Matthew Clark ,  Richard Wagner ,  David I. Israel ,  Malcolm L. Gefter ,  Dennis Benjamin ,  Nils Jakob Vest Hansen ,  Malcolm J. Kavarana ,  Steffen Phillip Creaser ,  George J. Franklin ,  Paulo A. Centrella ,  Raksha A. Acharya

发明人： Barry Morgan ,  Stephen Hale ,  Christopher C. Arico-Muendel ,  Matthew Clark ,  Richard Wagner ,  David I. Israel ,  Malcolm L. Gefter ,  Dennis Benjamin ,  Nils Jakob Vest Hansen ,  Malcolm J. Kavarana ,  Steffen Phillip Creaser ,  George J. Franklin ,  Paulo A. Centrella ,  Raksha A. Acharya

IPC分类号： C40B50/16

CPC分类号： C12N15/1068 ,  C07H21/00 ,  C40B50/10

摘要： The present invention provides methods of synthesizing libraries of molecules comprising a functional moiety which is operatively linked to an encoding oligonucleotide, wherein the encoding oligonucleotide comprises a capping sequence containing degenerate nucleotides. The methods generally include providing a solution comprising initiator compounds comprising an initial functional moiety comprising n building blocks which is operatively linked to an initial oligonucleotide; dividing the solution into reaction vessels; reacting the initiator compounds in each reaction vessel with a building block comprising a complementary reactive group to form a covalent bond; and reacting the initial oligonucleotide in each aliquot with a distinct incoming oligonucleotide in the presence of an enzyme which catalyzes the ligation of the incoming oligonucleotide and the initial oligonucleotide, under conditions suitable for enzymatic ligation of the incoming oligonucleotide and the initial oligonucleotide to form an encoding oligonucleotide.

摘要翻译： 本发明提供了合成包含与编码寡核苷酸有效连接的功能部分的分子文库的方法，其中编码寡核苷酸包含含有简并核苷酸的封端序列。 所述方法通常包括提供包含引发剂化合物的溶液，所述引发剂化合物包含初始功能部分，其包含可操作地连接于初始寡核苷酸的n 将溶液分解成反应容器; 使每个反应容器中的引发剂化合物与包含互补反应性基团的构建块反应形成共价键; 并且在适合于引入寡核苷酸和初始寡​​核苷酸的酶连接的条件下，在催化引入的寡核苷酸和初始寡​​核苷酸的连接的酶存在下，使各等分试样中的初始寡核苷酸与不同的入侵寡核苷酸反应，形成编码 寡核苷酸。