公开(公告)号：US10287620B2

公开(公告)日：2019-05-14

申请号：US15581401

申请日：2017-04-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Man Cheng ,  Xiangdong Meng ,  Jan Zur Megede

IPC: C12N15/62 ,  C12Q1/48

Abstract: Methods of determining polymerase fidelity are provided. In one embodiment, the method comprises filling a gapped plasmid with a polymerase to form a gap-filled plasmid, wherein the gap-filled plasmid comprises a gene encoding an protein that is functional or non-functional depending on the polymerase fidelity; forming a plurality of partitions from a solution comprising the gap-filled plasmid and a label for detecting the presence of the plasmid; detecting the presence of the gap-filled plasmid in one or more of the partitions; and determining the fidelity of the polymerase by determining a ratio of partitions containing the gene encoding a functional protein to partitions containing a gene encoding a non-functional protein.

公开(公告)号：US20180238865A1

公开(公告)日：2018-08-23

申请号：US15752108

申请日：2016-08-18

Applicant: Bio-Rad Laboratories, inc.

Inventor： Jan Zur Megede ,  George Karlin-Neumann

IPC: G01N33/542 ,  G01N33/58

CPC classification number: G01N33/542 ,  G01N33/581 ,  G01N33/582 ,  G01N2333/57 ,  G01N2333/575

Abstract: Methods of detecting an analyte in a sample are provided. In one embodiment, the method comprises forming a partition comprising the sample, a binding agent capable of emitting a signal when bound to the analyte, and a marker capable of identifying the partition; allowing the binding agent to bind to the analyte, if present; and detecting the presence of the analyte in the sample by detecting the signal emitted from the binding agent in the partition.