公开(公告)号：US10919930B2

公开(公告)日：2021-02-16

申请号：US15910702

申请日：2018-03-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Peter S. Gagnon

IPC: C07K1/18 ,  C07K1/16

Abstract: Methods are disclosed for use of apatite chromatography, particularly without reliance upon phosphate gradients, for purification or separation of at least one intact non-aggregated antibody, or at least one immunoreactive antibody fragment, from an impure preparation. Integration of such methods into multi-step procedures with other fractionation methods are additionally disclosed.

公开(公告)号：US09938317B2

公开(公告)日：2018-04-10

申请号：US14819334

申请日：2015-08-05

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Peter S. Gagnon

IPC: C07K1/18 ,  C07K1/16

CPC classification number: C07K1/18 ,  C07K1/16 ,  C07K1/165

Abstract: Methods are disclosed for use of apatite chromatography, particularly without reliance upon phosphate gradients, for purification or separation of at least one intact non-aggregated antibody, or at least one immunoreactive antibody fragment, from an impure preparation. Integration of such methods into multi-step procedures with other fractionation methods are additionally disclosed.

公开(公告)号：US20130267690A1

公开(公告)日：2013-10-10

申请号：US13622093

申请日：2012-09-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Peter S. Gagnon

IPC: C07K1/18

CPC classification number: C07K1/18 ,  A61L2/0088 ,  B01D15/3847 ,  C07K1/20 ,  C07K16/065 ,  C07K2317/52

Abstract: Methods, compositions and kits for chromatography purification of antibodies are provided. In some embodiments, antibodies are purified by hydroxyapatite (HT) or fluorapatite (FT) that is treated with a polycationic agent. In some embodiments, the antibodies are treated with a polycationic agent that is also a virucidal agent prior to purification.

Abstract translation: 提供了用于抗体的色谱纯化的方法，组合物和试剂盒。 在一些实施方案中，抗体通过用聚阳离子剂处理的羟基磷灰石（HT）或氟磷灰石（FT）进行纯化。 在一些实施方案中，抗体在纯化之前用也是杀病毒剂的聚阳离子剂处理。

公开(公告)号：US20180186831A1

公开(公告)日：2018-07-05

申请号：US15910702

申请日：2018-03-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Peter S. Gagnon

IPC: C07K1/18 ,  C07K1/16

CPC classification number: C07K1/18 ,  C07K1/16 ,  C07K1/165

Abstract: Methods are disclosed for use of apatite chromatography, particularly without reliance upon phosphate gradients, for purification or separation of at least one intact non-aggregated antibody, or at least one immunoreactive antibody fragment, from an impure preparation. Integration of such methods into multi-step procedures with other fractionation methods are additionally disclosed.

公开(公告)号：US20150353598A1

公开(公告)日：2015-12-10

申请号：US14819334

申请日：2015-08-05

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Peter S. Gagnon

IPC: C07K1/18

CPC classification number: C07K1/18 ,  C07K1/16 ,  C07K1/165

Abstract: Methods are disclosed for use of apatite chromatography, particularly without reliance upon phosphate gradients, for purification or separation of at least one intact non-aggregated antibody, or at least one immunoreactive antibody fragment, from an impure preparation. Integration of such methods into multi-step procedures with other fractionation methods are additionally disclosed.