公开(公告)号：US12077570B2

公开(公告)日：2024-09-03

申请号：US18118558

申请日：2023-03-07

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Andrew Tustian ,  Ankit Vartak ,  Thomas Daly ,  Erica Pyles ,  Nisha Palackal

IPC: C07K1/36 ,  A61K38/18 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K14/71 ,  C07K16/22 ,  C07K16/28 ,  C12N5/00 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/80 ,  G01N30/02

CPC classification number: C07K14/71 ,  A61K38/1866 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/22 ,  C07K16/283 ,  C12N5/0018 ,  C12N5/0031 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/80 ,  C07K2317/622 ,  C07K2319/30 ,  C07K2319/33 ,  C12N2800/10 ,  G01N2030/027

Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins.

公开(公告)号：US20240277859A1

公开(公告)日：2024-08-22

申请号：US18682046

申请日：2022-08-22

Applicant: Genentech, Inc.

Inventor： Rachel Hendricks ,  Matthew Henry Hutchinson ,  Mark Frederick Fedesco ,  Bejamin Phu Tran

IPC: A61K47/68 ,  C07K1/107 ,  C07K1/18

CPC classification number: A61K47/68 ,  C07K1/1077 ,  C07K1/18

Abstract: The present invention relates to a method of developing purification processes for antibody drug conjugates using cation-exchange chromatography in flow-through mode leveraging the purification conditions of the antibody intermediate without a change to the critical quality attributes (CQA) of the ADC.

公开(公告)号：US12060407B2

公开(公告)日：2024-08-13

申请号：US17025893

申请日：2020-09-18

Applicant: Cytune Pharma

Inventor： David Bechard ,  Guy De Martynoff

IPC: C07K14/705 ,  B01D15/14 ,  B01D15/32 ,  B01D15/36 ,  B01J41/20 ,  C07K1/18 ,  C07K1/20 ,  C07K14/54 ,  C07K14/715 ,  A61K38/00

CPC classification number: C07K14/7155 ,  B01D15/14 ,  B01D15/327 ,  B01D15/363 ,  B01J41/20 ,  C07K1/18 ,  C07K1/20 ,  C07K14/5443 ,  A61K38/00 ,  C07K2319/00 ,  B01D15/363 ,  B01D15/327

Abstract: The present invention relates to a method for preparing a composition comprising monomeric conjugates from a sample, said conjugate comprising (a) a polypeptide comprising the amino acid sequence of interleukin 15 or derivatives thereof, and (b) a polypeptide comprising the amino acid sequence of the sushi domain of IL-15Rα or derivatives thereof; wherein said method comprises the use of anion-exchange chromatography followed by a hydrophobic interaction chromatography; and to a pharmaceutical composition which can be obtained by such a method.

公开(公告)号：US12054532B2

公开(公告)日：2024-08-06

申请号：US17458519

申请日：2021-08-26

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Shunhai Wang ,  Ning Li ,  Hunter Chen ,  Amardeep Singh Bhupender Bhalla

IPC: C07K14/71 ,  A61K38/18 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/22 ,  C07K16/28 ,  C12N5/00 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/80 ,  G01N30/02

CPC classification number: C07K14/71 ,  A61K38/1866 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/22 ,  C07K16/283 ,  C12N5/0018 ,  C12N5/0031 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/80 ,  C07K2317/622 ,  C07K2319/30 ,  C07K2319/33 ,  C12N2800/10 ,  G01N2030/027

Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.

公开(公告)号：US12018075B2

公开(公告)日：2024-06-25

申请号：US16389055

申请日：2019-04-19

Applicant: Janssen Biotech, Inc.

Inventor： Paul Randolph

IPC: C07K16/24 ,  B01D15/16 ,  B01D15/20 ,  B01D15/36 ,  B01D15/38 ,  C07K1/18 ,  C07K1/22 ,  G01N30/56 ,  G01N30/86 ,  G01N35/00

CPC classification number: C07K16/244 ,  B01D15/166 ,  B01D15/20 ,  B01D15/206 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3809 ,  C07K1/18 ,  C07K1/22 ,  C07K16/241 ,  G01N30/56 ,  G01N30/8665 ,  G01N35/00623 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/565 ,  G01N2030/562

Abstract: A method of operating a chromatography column is described for use in methods of manufacture for producing anti-TNF antibodies, e.g., the anti-TNFα antibody SIMPONI® (golimumab). This method involves collecting column outlet signal and accumulated flow parameters at two or more intervals of at least one mobile phase transition front during operation of the chromatography column comprising column packing. A model gamma cumulative distribution curve is calculated based on the collected column outlet signal and accumulated flow parameters for the at least one mobile phase transition front. A height equivalent theoretical plate (HETP) value is calculated for the at least one mobile phase transition front using parameters of the model gamma cumulative distribution curve and the quality of the chromatography column packing is assessed based on the calculated HETP value.

公开(公告)号：US12018046B2

公开(公告)日：2024-06-25

申请号：US17718116

申请日：2022-04-11

Applicant: Biogen MA Inc.

Inventor： Austen Ng ,  Robert S. Gronke

IPC: C07K1/18 ,  C07K14/715 ,  C07K14/755

CPC classification number: C07K1/18 ,  C07K14/7151 ,  C07K14/755 ,  C07K2319/30

Abstract: The present invention relates to improved methods in the separation recombinant polypeptides with post-translational modifications from complex mixtures through the use of a cation exchange medium.

公开(公告)号：US11993630B2

公开(公告)日：2024-05-28

申请号：US17145653

申请日：2021-01-11

Applicant: Hoffmann-La Roche Inc.

Inventor： Roberto Falkenstein ,  Bernhard Spensberger

IPC: C07K1/16 ,  B01D15/26 ,  B01D15/36 ,  C07K1/18 ,  C07K14/505

CPC classification number: C07K1/18 ,  B01D15/362 ,  C07K14/505

Abstract: Herein is reported a method for the purification of a protein comprising erythropoietin and a single poly (ethylene glycol) residue from reaction by-products or not reacted starting material by a cation exchange chromatography method. It has been found that by employing a cation exchange Toyopearl® SP-650 chromatography material and employing a second wash step with an increased pH value compared to the first wash step a fusion protein of erythropoietin and a single poly (ethylene glycol) residue can be obtained in a single step with high purity and yield and suitability for large scale applications.

公开(公告)号：US11992825B2

公开(公告)日：2024-05-28

申请号：US15758012

申请日：2016-07-07

Applicant: Sartorius Stedim Biotech GmbH

Inventor： Louis Villain ,  Florian Taft ,  Jan Schwellenbach

IPC: B01J20/289 ,  B01D15/20 ,  B01J20/32 ,  C07K1/16 ,  C07K1/18 ,  C12N9/36 ,  B01D15/36

CPC classification number: B01J20/289 ,  B01D15/20 ,  B01J20/321 ,  B01J20/3219 ,  B01J20/3278 ,  B01J20/328 ,  B01J20/3293 ,  C07K1/16 ,  C07K1/18 ,  C12N9/2462 ,  B01D15/362

Abstract: The present invention relates to an adsorption medium including polymeric space elements which have been bonded to the surface of the chromatography matrix, and polymer chains containing chromatographically active centers, wherein the polymer chains have been bonded to the polymeric spacer elements, as well as to a method for the production thereof, and to the use of the adsorption medium for the purification of biomolecules.

公开(公告)号：US20240132566A1

公开(公告)日：2024-04-25

申请号：US17769630

申请日：2020-10-15

Applicant: JCR PHARMACEUTICALS CO., LTD.

Inventor： Shinji KAKIMOTO ,  Tsuyoshi FUKUI ,  Yukichi HATANO ,  Ayaka KOTANI ,  Ryota KATSUNO ,  Yuko OKADA

IPC: C07K14/61 ,  C07K1/18 ,  C07K1/22 ,  C07K14/765

CPC classification number: C07K14/61 ,  C07K1/18 ,  C07K1/22 ,  C07K14/765 ,  C07K2319/31

Abstract: Disclosed is a method for producing a fusion protein of human serum albumin and human growth hormone. The method comprises (a) a step of culturing a mammalian cell capable of producing the protein in a serum-free medium and causing the protein to be secreted into the culture fluid, (b) a step of collecting a culture supernatant by removing the mammalian cell from the culture fluid, and (c) a step of purifying the protein from the culture supernatant by using column chromatography using a material to which an antibody having an affinity for the protein is bound as a stationary phase, column chromatography using a material having an affinity for phosphate group as a stationary phase, cation exchange column chromatography, and size exclusion column chromatography.

公开(公告)号：US20240132537A1

公开(公告)日：2024-04-25

申请号：US18279170

申请日：2022-02-28

Applicant: XPRESS BIOLOGICS

Inventor： Marc DAUKANDT ,  Philippe LEDENT ,  Christian RODRIQUEZ

IPC: C07K1/18 ,  C07K1/36

CPC classification number: C07K1/18 ,  C07K1/36

Abstract: A method is for purifying a biomolecule (protein, DNA) of interest from a culture of Gram-negative bacteria uses polyoxyethylene isooctylcyclohexyl ether to remove endotoxins. Polyoxyethylene isooctylcyclohexyl ether is used to remove endotoxins and a solution having a pH between 8.0 and 9.0 and a concentration of salts from 2.5M to 3M in chromatography is used for specific leaching of bacterial contaminants.