公开(公告)号：US20170314060A1

公开(公告)日：2017-11-02

申请号：US15581401

申请日：2017-04-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Man CHENG ,  Xiangdong MENG ,  Jan ZUR MEGEDE

IPC: C12Q1/48

CPC classification number: C12Q1/485 ,  C12Q1/48 ,  G01N2333/91245 ,  G01N2333/9126 ,  G01N2333/9128

Abstract: Methods of determining polymerase fidelity are provided. In one embodiment, the method comprises filling a gapped plasmid with a polymerase to form a gap-filled plasmid, wherein the gap-filled plasmid comprises a gene encoding an protein that is functional or non-functional depending on the polymerase fidelity; forming a plurality of partitions from a solution comprising the gap-filled plasmid and a label for detecting the presence of the plasmid; detecting the presence of the gap-filled plasmid in one or more of the partitions; and determining the fidelity of the polymerase by determining a ratio of partitions containing the gene encoding a functional protein to partitions containing a gene encoding a non-functional protein.

公开(公告)号：US20190225951A1

公开(公告)日：2019-07-25

申请号：US16248442

申请日：2019-01-15

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Xiangdong MENG ,  Man CHENG

IPC: C12N9/12 ,  C12Q1/6876

Abstract: Mutant Type-A DNA polymerases having increased resistance to one or more polymerization activity inhibitors are provided.