公开(公告)号：US11353448B2

公开(公告)日：2022-06-07

申请号：US15045241

申请日：2016-02-16

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Min Xue ,  Wei Wei ,  James R. Heath

IPC: G01N33/53 ,  G01N33/573

Abstract: Methods and compositions for quantifying the cellular concentration of cyclic adenosine monophosphate (cAMP), cyclic guanine monophosphate (cGMP), glutathione, glucose, and glutamine in a single cell include the use of conjugates and analogs in immunofluorescent assays for on-chip quantification. These metabolite immunofluorescent assays may be incorporated with proteomic assays for simultaneous single cell analysis of metabolites and proteins.

公开(公告)号：US20160238594A1

公开(公告)日：2016-08-18

申请号：US15045241

申请日：2016-02-16

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Min Xue ,  Wei Wei ,  James R. Heath

IPC: G01N33/53

CPC classification number: G01N33/5308 ,  G01N33/573

Abstract: Methods and compositions for quantifying the cellular concentration of cyclic adenosine monophosphate (cAMP), cyclic guanine monophosphate (cGMP), glutathione, glucose, and glutamine in a single cell include the use of conjugates and analogs in immunofluorescent assays for on-chip quantification. These metabolite immunofluorescent assays may be incorporated with proteomic assays for simultaneous single cell analysis of metabolites and proteins.

Abstract translation: 用于量化单个细胞中环腺苷一磷酸（cAMP），环鸟嘌呤一磷酸（cGMP），谷胱甘肽，葡萄糖和谷氨酰胺的细胞浓度的方法和组合物包括在免疫荧光测定中使用缀合物和类似物进行片上定量。 这些代谢物免疫荧光测定可以与代谢物和蛋白质的同时单细胞分析的蛋白质组测定结合。