公开(公告)号：US11198898B2

公开(公告)日：2021-12-14

申请号：US16131798

申请日：2018-09-14

申请人： CORNELL UNIVERSITY

发明人： Jeremy Baskin ,  Timothy Bumpus ,  Dongjun Liang

IPC分类号： C12Q1/42 ,  C07C233/60 ,  C07C33/05 ,  C07C43/196 ,  C07C235/08 ,  C07D321/12 ,  C07D313/18

摘要： A method for detecting phospholipase D (PLD) activity in a cell, comprising: (i) stimulating endogenous PLD in said cell for said PLD to catalyze a transphosphatidylation reaction between phosphatidylcholine or a derivative thereof and an exogenous functionalized alcohol to form a phosphatidyl alcohol, wherein the functionalized alcohol possesses a first functional group that can react with and form a bond to a functionalized detectable label having a second functional group reactive with the first functional group, and said phosphatidyl alcohol contains said first functional group in available form; (ii) reacting said phosphatidyl alcohol with said functionalized detectable label under conditions where said functionalized detectable label reacts, via its second functional group, with the first functional group to form a linkage between said detectable label and said phosphatidyl alcohol so as to form a labeled phosphatidyl alcohol containing said detectable label; and (iii) detecting said labeled phosphatidyl alcohol.