公开(公告)号：US09540657B2

公开(公告)日：2017-01-10

申请号：US13901940

申请日：2013-05-24

Applicant: California Institute of Technology

Inventor： Kenneth Yu ,  David Baltimore ,  Lili Yang

IPC: C12N15/85 ,  C12N15/86 ,  C12N15/90 ,  G01N33/58 ,  G01N33/68

CPC classification number: C12N15/85 ,  C07K2319/00 ,  C12N15/86 ,  C12N15/907 ,  C12N2740/15043 ,  G01N33/582 ,  G01N33/68

Abstract: Some embodiments herein provide compositions and methods for expressing secreted and cell-surface-bound polypeptides in a single cell. In some embodiments, secreted and cell-surface polypeptide are produced from a single polynucleotide. The polynucleotide can comprise a sequence (or sequence encoding a polypeptide) that mediates separation of a membrane anchor from the polypeptide. In some embodiments, a desired ratio of secreted to surface-bound polypeptide is obtained by selecting a sequence that mediates a desired level of separation of the membrane anchor from the polypeptide.

Abstract translation: 本文的一些实施方案提供了用于在单个细胞中表达分泌的和细胞表面结合的多肽的组合物和方法。 在一些实施方案中，分泌的和细胞表面多肽由单个多核苷酸产生。 多核苷酸可以包含介导膜锚定体与多肽分离的序列（或编码多肽的序列）。 在一些实施方案中，分泌到表面结合多肽的期望比例通过选择介导膜锚与多肽分离的期望水平的序列来获得。