公开(公告)号：US11571635B2

公开(公告)日：2023-02-07

申请号：US15562586

申请日：2016-03-23

Applicant: Cytiva Sweden AB

Inventor： Bjorn Markus Olovsson ,  Anders Ljunglof ,  Karol Maciej Lacki

IPC: B01D15/00 ,  B01D15/38 ,  B01D15/18 ,  B01D15/14 ,  G01N30/46 ,  C07K1/22 ,  C12N7/00 ,  G01N30/20 ,  G01N30/88

Abstract: The present invention relates to a liquid chromatography system for the separation of bio-molecules in a fluid including at least two unit operations, wherein the first unit operation is a step of multi-column chromatography and the second unit operation is a step modifying said bio-molecules and/or the fluid, wherein the modification comprises feeding the fluid resulting from the last chromatography column of the first unit operation into a system comprising at least two containers, wherein each container has a volume and a moveable sidewall arranged to divide the volume into a first sub-volume and a second sub-volume, and each container comprises a first port connected to the first volume and a second port connected to the second sub-volume. The invention also relates to a virus inactivation device for a chromatography system according to the invention, which enables continuous or semi-continuous processing of biomolecules, as well as a method of using such a device.