公开(公告)号：US12280168B2

公开(公告)日：2025-04-22

申请号：US17437097

申请日：2020-03-23

Applicant: Cytiva Sweden AB

Inventor： Johan Avallin ,  Anna Gronberg ,  Anders Nilsson ,  Henrik Ingvarsson ,  Linda Persson ,  Magnus Asplund ,  Reinhard Braaz

IPC: A61L2/18 ,  B01D15/20 ,  B01D15/38 ,  G01N30/50 ,  G01N30/56 ,  G01N30/60 ,  A61L101/36

Abstract: The invention discloses a method for sanitization of a bioprocess chromatography column, comprising the steps of: a) providing a bioprocess chromatography column with a packed bed of separation resin particles and a movable adapter, b) with the adapter in contact with the packed bed, conveying a sanitization fluid through the packed bed to a column outlet; c) raising the adapter to provide a gap between the packed bed and the adapter, d) lowering the adapter to close the gap; and e) conveying an equilibration liquid through the packed bed to a column outlet.

公开(公告)号：US20220184527A1

公开(公告)日：2022-06-16

申请号：US17686157

申请日：2022-03-03

Applicant: Cytiva Sweden AB

Inventor： Johan Avallin ,  Klaus Gebauer

IPC: B01D15/20 ,  A61L2/08 ,  G01N30/56

Abstract: A method and a system and a container system for transferring separation resin from at least one first container (3; 3′; 3a, 3b; 3a′, 3b′, 3c′, 3d′) to a second container (5; 5′), wherein said first container is a deformable, single-use separation resin storage container, said method comprising the steps of:
preparing (S1) the at least one first container by providing a deformable, single-use container comprising an outlet port (4′) with a predefined volume of separation resin in a storage solution;
fluidizing (S3) the separation resin in the at least one first container to provide a resin slurry, said fluidizing being performed by mechanical interaction to the first container from an outside of the first container to provide a deformation of said first container;
fluidically connecting (S5) the outlet port (4′) of the at least one first container to an inlet port (103) of the second container;
transferring (S7) separation resin from the at least one first container to the second container by generating a pressure difference between an interior of the second container and an interior of the first container where the pressure is lower in the second container.

公开(公告)号：US20220175992A1

公开(公告)日：2022-06-09

申请号：US17437097

申请日：2020-03-23

Applicant: Cytiva Sweden AB

Inventor： Johan Avallin ,  Anna Gronberg ,  Anders Nilsson ,  Henrik Ingvarsson ,  Linda Persson ,  Magnus Asplund ,  Reinhard Braaz

IPC: A61L2/18 ,  B01D15/20 ,  B01D15/38 ,  G01N30/50 ,  G01N30/60 ,  G01N30/56

Abstract: The invention discloses a method for sanitization of a bioprocess chro matography column, comprising the steps of: a) providing a bioprocess chromatogra phy column with a packed bed of separation resin particles and a movable adapter, b) with the adapter in contact with the packed bed, conveying a sanitization fluid through the packed bed to a column outlet; c) raising the adapter to provide a gap between the packed bed and the adapter, d) lowering the adapter to close the gap; and e) conveying an equilibration liquid through the packed bed to a column outlet.

公开(公告)号：US11291929B2

公开(公告)日：2022-04-05

申请号：US16481655

申请日：2018-01-30

Applicant: Cytiva Sweden AB

Inventor： Johan Avallin ,  Klaus Gebauer

IPC: B01D15/20 ,  G01N30/56 ,  A61L2/08

Abstract: A method and a system and a container system for transferring separation resin from at least one first container (3; 3; 3a, 3b; 3a′, 3b′, 3c′, 3d′) to a second container (5; 5′), wherein said first container is a deformable, single-use separation resin storage container, said method comprising the steps of:—preparing (S1) the at least one first container by providing a deformable, single-use container comprising an outlet port (4′) with a predefined volume of separation resin in a storage solution;—fluidizing (S3) the separation resin in the at least one first container to provide a resin slurry, said fluidizing being performed by mechanical interaction to the first container from an outside of the first container to provide a deformation of said first container;—fluidically connecting (S5) the outlet port (4′) of the at least one first container to an inlet port (103) of the second container;—transferring (S7) separation resin from the at least one first container to the second container by generating a pressure difference between an interior of the second container and an interior of the first container where the pressure is lower in the second container.