公开(公告)号：US09994837B2

公开(公告)日：2018-06-12

申请号：US15110360

申请日：2015-01-13

Applicant: DSM IP ASSETS B.V.

Inventor： René Marcel De Jong ,  Ulrike Maria Müller ,  Petrus Jacobus Theodorus Dekker

IPC: C12N9/38 ,  A23L29/00 ,  A23C9/152

CPC classification number: C12N9/2471 ,  A23C9/1526 ,  A23L29/06 ,  A23V2002/00 ,  C12Y302/01107 ,  C12Y302/01108

Abstract: The invention relates to a variant polypeptide having lactase activity. The invention also relates to a nucleic acid sequence encoding such a variant polypeptide, to a nucleic acid construct comprising said nucleic acid sequence, to a recombinant expression vector comprising said nucleic acid construct and to a recombinant host cell comprising said expression vector. Further, the invention relates to a method for producing a lactase variant via use of such a host cell. Also, the invention relates to a method of producing a lactase polypeptide variant. The invention further relates to a composition comprising a lactase variant, to use of such a lactase variant or to the use of a lactase variant-containing composition in the preparation of a dairy product, to a process for the production of a dairy product and to the resulting dairy product.