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公开(公告)号：US09994837B2
公开(公告)日：2018-06-12
申请号：US15110360
申请日：2015-01-13
Applicant: DSM IP ASSETS B.V.
CPC classification number: C12N9/2471 , A23C9/1526 , A23L29/06 , A23V2002/00 , C12Y302/01107 , C12Y302/01108
Abstract: The invention relates to a variant polypeptide having lactase activity. The invention also relates to a nucleic acid sequence encoding such a variant polypeptide, to a nucleic acid construct comprising said nucleic acid sequence, to a recombinant expression vector comprising said nucleic acid construct and to a recombinant host cell comprising said expression vector. Further, the invention relates to a method for producing a lactase variant via use of such a host cell. Also, the invention relates to a method of producing a lactase polypeptide variant. The invention further relates to a composition comprising a lactase variant, to use of such a lactase variant or to the use of a lactase variant-containing composition in the preparation of a dairy product, to a process for the production of a dairy product and to the resulting dairy product.
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公开(公告)号：US12049658B2
公开(公告)日：2024-07-30
申请号：US16648879
申请日：2018-09-25
Applicant: DSM IP ASSETS B.V.
Inventor： Nathalie Balch , Paul Blomquist , Reed Doten , Peter Houston , Ethan Lam , Jenna Mcmahon , Joshua Trueheart , Celine Viarouge , René Marcel De Jong
IPC: C12P23/00
CPC classification number: C12P23/00
Abstract: The present invention is related to production of retinyl esters, such as in particular retinyl acetate, an important building block for production of vitamin A. The retinyl esters might be generated via enzymatic conversion of retinol which process includes the use of enzymes with acetyl-transferase (ATF) activity, thus acetylating retinol into retinol/retinyl acetate. Said process is particularly useful for biotechnological production of vitamin A.
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公开(公告)号：US11944104B2
公开(公告)日：2024-04-02
申请号：US17045062
申请日：2019-04-04
Applicant: DSM IP ASSETS B.V.
Inventor： Joke Anneleen Putseys , Aloysius Wilhelmus Rudolphus Hubertus Teunissen , René Marcel De Jong , Jeroen Godefrooij , Helma Arina Stolze-Lagerweij
CPC classification number: A21D8/042 , C12N9/2414 , C12Y302/01133
Abstract: The present invention relates to a variant polypeptide having maltogenic alpha-amylase activity wherein the polypeptide comprises an amino acid sequence, which, when aligned with an amino acid sequence of SEQ ID NO: 1, comprises an amino acid substitution F188L/I, S200N, and D261G, and optionally a further amino acid substitution T288P, wherein the amino acid substitutions are determined with reference to SEQ ID NO: 1, and wherein the polypeptide has an amino acid sequence which has at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or at least 99% identity to the amino acid sequence of SEQ ID NO: 1. The invention also relates to a process for the preparation of a dough or a baked product wherein the variant polypeptide is used.
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公开(公告)号：US20150140169A1
公开(公告)日：2015-05-21
申请号：US14397865
申请日：2013-05-03
Applicant: DSM IP ASSETS B.V.
Inventor： Petrus Jacobus Theodorus Dekker , René Marcel De Jong , Michael Dennis Tabeling , Cornelis Marinus Muijlwijk
IPC: C12N9/64 , A23C19/032
CPC classification number: C12N9/6483 , A23C19/032 , A23C19/0326 , A23C19/0684 , A23C19/072 , A23C2220/202 , A23C2250/15 , C12Y304/23004
Abstract: The invention relates to a polypeptide having chymosin activity which: a. is capable of hydrolysing bovine alpha s1-casein at position F23F24 so as to form αs1-I CN (f24-199) more rapidly than camel chymosin; and b. has a C/P ratio higher than the C/P ratio of bovine chymosin.
Abstract translation: 本发明涉及具有凝乳酶活性的多肽，其具有：a。 能够在F23F24位水解牛α1酪蛋白，从而比骆驼凝乳酶更快地形成αs1-CN（f24-199）; 和b。 C / P比高于牛乳蛋白的C / P比。
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Patent Agency Ranking
公开(公告)号：US12195769B2
公开(公告)日：2025-01-14
申请号：US17253266
申请日：2019-06-18
Applicant: DSM IP ASSETS B.V.
Inventor： Joke Anneleen Putseys , Neil Carr , René Marcel De Jong , Aloysius Wilhelmus Rudolphus Hubertus Teunissen
Abstract: The present invention relates to a variant polypeptide having a lipase activity, wherein the polypeptide may be a polypeptide that has an amino acid sequence which has at least 70% identity to a mature amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 6, wherein the polypeptide comprises an amino acid substitution at position 295 and at least one further amino acid substitution at position 113, 121, 179 and/or 284, wherein the positions are defined with reference to SEQ ID NO: 1 or SEQ ID NO: 6. The invention further relates to a process for preparing a dough wherein a polypeptide as disclosed herein is used and baked product prepared from the dough.
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公开(公告)号：US11608364B2
公开(公告)日：2023-03-21
申请号：US17051299
申请日：2019-04-29
Applicant: DSM IP ASSETS B.V.
Inventor： Johannes Gustaaf Ernst Van Leeuwen , Viktor Marius Boer , Priscilla Zwartjens , Jos Van Vugt , René Marcel De Jong
Abstract: The disclosure provides a recombinant cell capable of producing a steviol glycoside, wherein the cell comprises a nucleic acid coding for a variant of a parent polypeptide, wherein the variant has steviol glycoside transport mediating activity, wherein the variant comprises an amino acid sequence which, when aligned with the amino acid sequence of the parent polypeptide, comprises at least one modification of the amino acid residue corresponding to any of the amino acids in the amino acid sequence of the parent polypeptide, wherein the variant has an improved ability to produce rebaudioside M and optionally other steviol glycosides extracellularly if compared with the parent polypeptide when measured under the same conditions.
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公开(公告)号：US12091436B2
公开(公告)日：2024-09-17
申请号：US18167119
申请日：2023-02-10
Applicant: DSM IP ASSETS B.V.
Inventor： Johannes Gustaaf Ernst Van Leeuwen , Viktor Marius Boer , Priscilla Zwartjens , Jos Van Vugt , René Marcel De Jong
CPC classification number: C07K14/39 , C12N9/0042 , C12P19/56 , C12Y106/02004
Abstract: The disclosure provides a recombinant cell capable of producing a steviol glycoside, wherein the cell comprises a nucleic acid coding for a variant of a parent polypeptide, wherein the variant has steviol glycoside transport mediating activity, wherein the variant comprises an amino acid sequence which, when aligned with the amino acid sequence of the parent polypeptide, comprises at least one modification of the amino acid residue corresponding to any of the amino acids in the amino acid sequence of the parent polypeptide, wherein the variant has an improved ability to produce rebaudioside M and optionally other steviol glycosides extracellularly if compared with the parent polypeptide when measured under the same conditions.
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公开(公告)号：US09944905B2
公开(公告)日：2018-04-17
申请号：US15103237
申请日：2014-12-12
Applicant: DSM IP ASSETS B.V.
Inventor： René Marcel De Jong , Zheng Zhao , Ben Den Dulk , Remko Tsjibbe Winter
IPC: C12P7/62 , C12P7/46 , C12N9/02 , C12P19/34 , C12P21/06 , C12N15/00 , C12N1/00 , C07H21/04 , C07K1/00
CPC classification number: C12N9/001 , C12P7/46 , C12Y103/01006
Abstract: The present invention relates to a variant polypeptide having fumarate reductase activity, which has modified NADP(H)-dependent and/or NAD(H)-dependent activity as compared with a reference polypeptide having fumarate reductase activity. Such a variant may be overexpressed in a host cell in order to improve production of a dicarboxylic acid.
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公开(公告)号：US20150118356A1
公开(公告)日：2015-04-30
申请号：US14397866
申请日：2013-05-03
Applicant: DSM IP ASSETS B.V.
IPC: A23C19/032 , C12N9/64
CPC classification number: C12N9/6483 , A23C19/032 , A23C19/0326 , A23C19/0684 , A23C19/072 , A23C2220/202 , A23C2250/15 , C12Y304/23004
Abstract: A variant polypeptide having chymosin activity, wherein the variant has an amino acid sequence which, when aligned with the chymosin comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue corresponding to any of amino acids 2, 22, 40, 48, 50, 51, 53, 61, 62, 76, 88, 98, 99, 109, 112, 117, 125, 126, 135, 144, 160, 161, 163, 187, 189, 194, 200, 201, 202, 203, 221, 223, 240, 242, 244, 254, 267, 271, 273, 278, 280, 284, 289, 292, 294 or 295 said positions being defined with reference to SEQ ID NO: 2 and wherein the variant has one or more altered properties as compared with a reference polypeptide having chymosin activity. Such a variant polypeptide may be used in the preparation of a cheese.
Abstract translation: 具有凝乳酶活性的变体多肽，其中所述变体具有当与包含SEQ ID NO：2所示的序列的凝乳酶比对时的氨基酸序列包含对应于任何氨基酸的氨基酸残基的至少一个取代 2，22，40，48，50，51，53，61，62，76，88，98，99，109，112，117，125，126，135，144，160，161，163，187， 194,2200,201,202,203,221,223,240,242,244,254,267,271,273,278,280,284,289,292,294或295表示所述位置是参照SEQ ID NO：2，其中与具有凝乳酶活性的参照多肽相比，变体具有一个或多个改变的特性。 这种变体多肽可用于制备奶酪。
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