公开(公告)号：US20110177602A1

公开(公告)日：2011-07-21

申请号：US13000385

申请日：2009-06-24

申请人： Dietmar Appelhans ,  Mathias Lakatos ,  Wolfgang Pompe

发明人： Dietmar Appelhans ,  Mathias Lakatos ,  Wolfgang Pompe

IPC分类号： G01N31/22 ,  B82Y15/00

CPC分类号： G01N33/587 ,  B82Y5/00 ,  G01N33/54346 ,  G01N33/54373 ,  G01N2333/395 ,  Y10T436/107497

摘要： A composite structure, for marking biomolecules in a biological, biochemical or medicinal system, comprises: at least one nano particle and at least one dendritic macromolecule, which has an inner region with branched, especially perfectly branched to highly branched, structures and a periphery, which comprises surface groups of the dendritic macromolecule, wherein a plurality, especially more than 50%, of the surface groups have in the periphery of the dendritic macromolecule, in each case, at least one functional group of first type, wherein the functional group of first type comprises at least one monosaccharide-, oligosaccharide- and/or polysaccharide unit, and wherein the dendritic macromolecule stabilizes the nano particle.

摘要翻译： 用于在生物，生物化学或药物系统中标记生物分子的复合结构包括：至少一个纳米颗粒和至少一个树突状大分子，其具有带有支链，特别是完全分支到高度支化的结构和外围的内部区域， 其包括树枝状高分子的表面基团，其中多个，特别是大于50％的表面基团在树突状高分子的周围具有至少一种第一类型的官能团，其中所述官能团为 第一类型包含至少一种单糖，寡糖和/或多糖单元，并且其中所述树突状高分子使纳米颗粒稳定。

公开(公告)号：US20100285505A1

公开(公告)日：2010-11-11

申请号：US12666299

申请日：2008-06-27

申请人： Kai Ostermann ,  Wolfgang Pompe ,  Dagmar Wersing ,  Mathias Lakatos ,  Michael Mertig ,  Gerhard Rödel ,  Simone Thierfelder

发明人： Kai Ostermann ,  Wolfgang Pompe ,  Dagmar Wersing ,  Mathias Lakatos ,  Michael Mertig ,  Gerhard Rödel ,  Simone Thierfelder

IPC分类号： G01N33/53 ,  C12M1/34

CPC分类号： G01N33/587 ,  G01N33/54373 ,  G01N2333/395

摘要： The invention relates to devices and methods for detecting a substance by cell surface polarizations and the detection thereof by means of PPR or particle-mediated fluorescence. The device for detecting a substance by cell surface polarization according to the invention has cells, wherein a gene the expression of which leads to the polarized presentation of a protein on the surface of the cell is placed under the control of a promotor which can be regulated by the substance to be detected, nanoparticles which are functionalized with a molecule which can bind specifically to the surface-exposed protein, and at least one optical measurement device, such that an accumulation of the nanoparticles on the surface of the cells can be detected by particle plasmon resonance or particle-mediated fluorescence.

摘要翻译： 本发明涉及用于通过细胞表面极化检测物质的装置和方法，并且其通过PPR或颗粒介导的荧光进行检测。 根据本发明的用于通过细胞表面极化检测物质的装置具有细胞，其中表达导致细胞表面上的蛋白质的极化表达的基因置于可调节的启动子的控制下 通过待检测的物质，用能够与表面暴露蛋白特异性结合的分子官能化的纳米颗粒和至少一种光学测量装置，使得纳米颗粒在细胞表面上的积聚可以通过 颗粒等离子体共振或粒子介导的荧光。