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公开(公告)号:US20240368639A1
公开(公告)日:2024-11-07
申请号:US18651609
申请日:2024-04-30
Inventor: Xiaozhou ZHANG , Jay PATEL
Abstract: Systems, methods, and compositions for biocatalyzed conversion of oleic acid to non-racemic (R)-10-hydroxystearic acid ((R)-10-HSA) are described herein. Some systems and methods may include mixing oleic acid, a buffer, and a surfactant to produce an emulsion. The emulsion may be mixed with recombinant enzyme produced by a transformed microorganism. The recombinant enzyme may biocatalytically convert oleic acid to (R)-10-HSA, which may be extracted from the emulsion via an organic solvent.