公开(公告)号：US20110283374A1

公开(公告)日：2011-11-17

申请号：US13146977

申请日：2010-01-29

申请人： Hiromitsu Nakauchi ,  Toshihiro Kobayashi ,  Tomoyuki Yamaguchi ,  Sanae Hamanaka ,  Masumi Hirabayashi ,  Megumi Kato

发明人： Hiromitsu Nakauchi ,  Toshihiro Kobayashi ,  Tomoyuki Yamaguchi ,  Sanae Hamanaka ,  Masumi Hirabayashi ,  Megumi Kato

IPC分类号： A01K67/027

CPC分类号： C12N15/8775 ,  A01K2267/025 ,  C12N15/877

摘要： A heterologous chimeric animal can be produced by a method comprising the steps of: (A) injecting a stem cell into a blastocyst cavity in a blastocyst stage of an animal heterologous to that of the stem cell, or mixing the stem cell with a divided fertilized egg of the animal heterologous to that of the stem cell; and (B) growing a cell mass including the stem cell prepared in the step (A) into a chimeric animal between a species of the stem cell and a species of the heterologous animal.

摘要翻译： 异源嵌合动物可以通过包括以下步骤的方法产生：（A）将干细胞注射到与干细胞异源的动物的胚泡期的胚泡腔中，或将干细胞与分配的受精 动物的卵与干细胞的异源; 和（B）将包含步骤（A）中制备的干细胞的细胞团生长成干细胞种和异源动物物种之间的嵌合动物。

公开(公告)号：US20100122360A1

公开(公告)日：2010-05-13

申请号：US12583559

申请日：2009-08-21

申请人： Hiromitsu Nakauchi ,  Toshihiro Kobayashi ,  Younsu Lee ,  Joichi Usui ,  Tomoyuki Yamaguchi ,  Sanae Hamanaka

发明人： Hiromitsu Nakauchi ,  Toshihiro Kobayashi ,  Younsu Lee ,  Joichi Usui ,  Tomoyuki Yamaguchi ,  Sanae Hamanaka

IPC分类号： A01K67/00 ,  C12N5/07 ,  A01N1/02

CPC分类号： A01K67/0271 ,  A01K2207/20 ,  A61L27/3834

摘要： An object of the present invention is to produce a mammalian organ having a complicated cellular composition composed of multiple kinds of cells, such as kidney, pancreas, thymus and hair, in the living body of a non-human animal. The inventors of the present invention applied the chimeric animal assay described above, to a novel solid organ production method. More specifically, the inventors has shown that a model mouse which is deficient of kidney, pancreas, thymus or hair due to the dysfunction of the metanephric mesenchyme that is differentiated into most of an adult kidney, is rescued by blastocyst complementation by the chimeric animal assay, and whereby a kidney, a pancreas, thymus or hair can be newly produced.

摘要翻译： 本发明的目的是在非人动物的活体中制备由多种细胞如肾，胰腺，胸腺和毛发组成的细胞组成复杂的哺乳动物器官。 本发明的发明人将上述嵌合动物实验应用于新型的固体器官生产方法。 更具体地，本发明人已经表明，由分化成大部分成年肾脏的间质间质的功能障碍引起的肾，胰腺，胸腺或毛发缺陷的模型小鼠通过嵌合动物试验通过囊胚互补来拯救 ，由此可以新生产肾，胰腺，胸腺或毛发。

公开(公告)号：US20110258715A1

公开(公告)日：2011-10-20

申请号：US13059941

申请日：2009-08-21

申请人： Hiromitsu Nakauchi ,  Toshihiro Kobayashi ,  Tomoyuki Yamaguchi ,  Sanae Hamanaka

发明人： Hiromitsu Nakauchi ,  Toshihiro Kobayashi ,  Tomoyuki Yamaguchi ,  Sanae Hamanaka

IPC分类号： A01K67/027 ,  A01K67/00 ,  C12N15/87

CPC分类号： A01K67/0271 ,  A01K67/0275 ,  A01K67/0276 ,  A01K2207/12 ,  A01K2227/105 ,  A01K2267/025 ,  A61L27/3834 ,  A61L27/3839 ,  A61L27/3895

摘要： It is revealed that an organ such as pancreas can be regenerated by utilizing a fact that the deficiency of an organ is complemented by injecting an induced pluripotent stem cell (iPS cell) into a developed blastocyst in a blastocyst complementation method. Thus, the present invention has solved the above-described object. This provides a method for producing a target organ, using an iPS cell, in a living body of a non-human mammal having an abnormality associated with a lack of development of the target organ in a development stage, the target organ produced being derived from a different individual mammal that is an individual different from the non-human mammal.

摘要翻译： 通过利用囊胚互补方法将发生的多能干细胞（iPS细胞）注射到发育的囊胚中，通过利用器官的不足补充了这一事实，能够再生胰脏等器官。 因此，本发明解决了上述目的。 这提供了使用iPS细胞在发育阶段中与目标器官缺乏发育相关的异常的非人哺乳动物的活体中产生靶器官的方法，所产生的靶器官来自 作为与非人哺乳动物不同的个体的不同个体哺乳动物。