公开(公告)号：US20240082461A1

公开(公告)日：2024-03-14

申请号：US18239036

申请日：2023-08-28

Applicant: The University of North Carolina at Chapel Hill

Inventor： Lola M. Reid ,  Wencheng Zhang ,  Eliane Wauthier

IPC: A61L27/38 ,  A61L27/20 ,  A61L27/22 ,  A61L27/26 ,  A61L27/34 ,  A61L27/36 ,  A61L27/52 ,  A61L27/56 ,  A61L27/58 ,  C12N5/071 ,  C12N5/0775

CPC classification number: A61L27/3886 ,  A61L27/20 ,  A61L27/227 ,  A61L27/26 ,  A61L27/34 ,  A61L27/3604 ,  A61L27/3641 ,  A61L27/3813 ,  A61L27/3834 ,  A61L27/3839 ,  A61L27/52 ,  A61L27/56 ,  A61L27/58 ,  C12N5/0662 ,  C12N5/0668 ,  C12N5/067 ,  C12N5/0676 ,  A61L2430/26 ,  C12N2506/1346

Abstract: Compositions and methods of transplanting cells by grafting strategies into solid organs (especially internal organs) are provided. These methods and compositions can be used to repair diseased organs or to establish models of disease states in experimental hosts. The method involves attachment onto the surface of a tissue or organ, a patch graft, a “bandaid-like” covering, containing epithelial cells with supporting early lineage stage mesenchymal cells. The cells are incorporated into soft gel-forming biomaterials prepared under serum-free, defined conditions comprised of nutrients, lipids, vitamins, and regulatory signals that collectively support stemness of the donor cells. The graft is covered with a biodegradable, biocompatible, bioresorbable backing used to affix the graft to the target site. The cells in the graft migrate into and throughout the tissue such that within a couple of weeks they are uniformly dispersed within the recipient (host) tissue. The mechanisms by which engraftment and integration of donor cells into the organ or tissue involve multiple membrane-associated and secreted forms of MMPs.

公开(公告)号：US11857697B2

公开(公告)日：2024-01-02

申请号：US15994749

申请日：2018-05-31

Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN

Inventor： Jason Spence ,  Alyssa Miller

IPC: C12N5/071 ,  C12N5/00 ,  A61K35/42 ,  A61L27/38 ,  C12N5/074 ,  C12N5/0735

CPC classification number: A61L27/3895 ,  A61K35/42 ,  A61L27/3804 ,  A61L27/3813 ,  A61L27/3834 ,  A61L27/3839 ,  A61L27/3869 ,  C12N5/0018 ,  C12N5/0062 ,  C12N5/0606 ,  C12N5/0607 ,  C12N5/0688 ,  C12N5/0689 ,  A61L2430/22 ,  C12N2501/113 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/119 ,  C12N2501/155 ,  C12N2501/385 ,  C12N2501/415 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/03 ,  C12N2506/27 ,  C12N2506/45 ,  C12N2513/00

Abstract: The invention disclosed herein generally relates to methods and systems for growing, expanding and/or obtaining 3-dimensional lung-like epithelium comprising cells having SOX9 protein activity and SOX2+ protein activity. In particular, the invention disclosed herein relates to methods and systems for growing human cells having SOX9 protein activity and SOX2+ protein activity in vitro, and for promoting pluripotent stem cell derived ventral-anterior foregut spheroid tissue into 3-dimensional lung-like epithelium comprising cells having SOX9 protein activity and SOX2+ protein activity.

公开(公告)号：US11672831B2

公开(公告)日：2023-06-13

申请号：US15464149

申请日：2017-03-20

Applicant: Takeda Pharmaceutical Company Limited ,  Universidad Autónoma de Madrid

Inventor： María Gema Fernández Miguel ,  Manuel Ángel González De La Peña ,  Rosa Ana García Castro ,  Mariano García Arranz ,  Damián García Olmo

IPC: C12N5/0775 ,  A61K35/35 ,  A61L24/10 ,  A61L27/36 ,  A61L27/38 ,  A61B17/16 ,  A61K45/06 ,  A61L24/00 ,  A61B17/32 ,  A61K35/12

CPC classification number: A61K35/35 ,  A61B17/1659 ,  A61B17/32 ,  A61K45/06 ,  A61L24/0015 ,  A61L24/106 ,  A61L27/3604 ,  A61L27/3834 ,  A61L27/3839 ,  A61L27/3869 ,  A61L27/3895 ,  C12N5/0667 ,  A61B2017/320008 ,  A61K35/12 ,  A61L2300/64 ,  C12N2509/00 ,  C12N2510/00 ,  C12N2533/56

Abstract: Provided herein are novel methods and composition utilizing adipose tissue-derived stromal stem cells for treating fistulae.

公开(公告)号：US20180353653A1

公开(公告)日：2018-12-13

申请号：US16006504

申请日：2018-06-12

Applicant: The University of North Carolina at Chapel Hill

Inventor： Lola M. Reid ,  Wencheng Zhang ,  Eliane Wauthier

IPC: A61L27/38 ,  A61L27/54 ,  A61L27/52 ,  A61L27/58 ,  A61L27/56 ,  A61L27/36 ,  A61L27/20 ,  A61L27/26 ,  A61F2/04

CPC classification number: A61L27/3886 ,  A61F2/04 ,  A61F2002/045 ,  A61L27/20 ,  A61L27/227 ,  A61L27/26 ,  A61L27/3604 ,  A61L27/3641 ,  A61L27/3808 ,  A61L27/3813 ,  A61L27/3834 ,  A61L27/3839 ,  A61L27/52 ,  A61L27/54 ,  A61L27/56 ,  A61L27/58 ,  A61L2430/22 ,  A61L2430/26 ,  A61L2430/28 ,  A61L2430/34 ,  A61P1/00 ,  A61P1/16 ,  A61P1/18 ,  A61P13/12

Abstract: Compositions and methods of transplanting cells by grafting strategies into solid organs (especially internal organs) are provided. These methods and compositions can be used to repair diseased organs or to establish models of disease states in experimental hosts. The method involves attachment onto the surface of a tissue or organ, a patch graft, a “bandaid-like” covering, containing epithelial cells with supporting early lineage stage mesenchymal cells. The cells are incorporated into soft gel-forming biomaterials prepared under serum-free, defined conditions comprised of nutrients, lipids, vitamins, and regulatory signals that collectively support stemness of the donor cells. The graft is covered with a biodegradable, biocompatible, bioresorbable backing used to affix the graft to the target site. The cells in the graft migrate into and throughout the tissue such that within a couple of weeks they are uniformly dispersed within the recipient (host) tissue. The mechanisms by which engraftment and integration of donor cells into the organ or tissue involve multiple membrane-associated and secreted forms of MMPs.

公开(公告)号：US20180353651A1

公开(公告)日：2018-12-13

申请号：US16006460

申请日：2018-06-12

Applicant: The University of North Carolina at Chapel Hill

Inventor： Lola M. Reid ,  Wencheng Zhang ,  Eliane Wauthier

IPC: A61L27/38 ,  A61L27/52 ,  A61L27/20 ,  A61L27/22 ,  A61L27/36

CPC classification number: A61L27/3886 ,  A61F2/04 ,  A61F2002/045 ,  A61L27/20 ,  A61L27/227 ,  A61L27/26 ,  A61L27/3604 ,  A61L27/3641 ,  A61L27/3808 ,  A61L27/3813 ,  A61L27/3834 ,  A61L27/3839 ,  A61L27/52 ,  A61L27/54 ,  A61L27/56 ,  A61L27/58 ,  A61L2430/22 ,  A61L2430/26 ,  A61L2430/28 ,  A61L2430/34 ,  A61P1/00 ,  A61P1/16 ,  A61P1/18 ,  A61P13/12

Abstract: Compositions and methods of transplanting cells by grafting strategies into solid organs (especially internal organs) are provided. These methods and compositions can be used to repair diseased organs or to establish models of disease states in experimental hosts. The method involves attachment onto the surface of a tissue or organ, a patch graft, a “bandaid-like” covering, containing epithelial cells with supporting early lineage stage mesenchymal cells. The cells are incorporated into soft gel-forming biomaterials prepared under serum-free, defined conditions comprised of nutrients, lipids, vitamins, and regulatory signals that collectively support stemness of the donor cells. The graft is covered with a biodegradable, biocompatible, bioresorbable backing used to affix the graft to the target site. The cells in the graft migrate into and throughout the tissue such that within a couple of weeks they are uniformly dispersed within the recipient (host) tissue. The mechanisms by which engraftment and integration of donor cells into the organ or tissue involve multiple membrane-associated and secreted forms of MMPs.

公开(公告)号：US20180305671A1

公开(公告)日：2018-10-25

申请号：US15769139

申请日：2016-10-21

Applicant: INDIANA UNIVERSITY RESEARCH AND TECHNOLOGY CORPORATION

Inventor： Karl R. Koehler ,  Jiyoon Lee

IPC: C12N5/071 ,  A61L27/52 ,  A61L27/56 ,  A61L27/54 ,  A61L27/58 ,  C12Q1/6876 ,  A61L27/38 ,  A61L27/36 ,  A61L27/60

CPC classification number: C12N5/0698 ,  A61K35/36 ,  A61L27/362 ,  A61L27/3633 ,  A61L27/3641 ,  A61L27/3687 ,  A61L27/3813 ,  A61L27/3834 ,  A61L27/3839 ,  A61L27/3891 ,  A61L27/3895 ,  A61L27/52 ,  A61L27/54 ,  A61L27/56 ,  A61L27/58 ,  A61L27/60 ,  A61L2300/412 ,  A61L2300/604 ,  A61L2300/64 ,  A61L2430/34 ,  A61L2430/40 ,  C12N5/0606 ,  C12N5/0629 ,  C12N2501/155 ,  C12N2501/999 ,  C12N2506/03 ,  C12N2506/1346 ,  C12N2513/00 ,  C12N2533/76 ,  C12Q1/6876 ,  C12Q2600/136

Abstract: Provided herein are methods for directing differentiation of human pluripotent stem cells into a three-dimensional multilayered skin composition comprising an epidermal layer, a dermal layer, and a plurality of cells capable of forming a functional hair follicle. Also provided herein are three-dimensional, multilayered engineered skin compositions and methods of using the same for drug screening, for screening compounds for effects on hair growth, and for other applications.

公开(公告)号：US10004602B2

公开(公告)日：2018-06-26

申请号：US15162492

申请日：2016-05-23

Applicant: INSTITUT QUIMIC DE SARRIA ,  UNIVERSITAD POLITECNICA DE VALENCIA ,  FUNDACIO GERMANS TRIAS PUJOL ,  ASSOCIATION CARDIO-MONDE

Inventor： Juan Carlos Chachques ,  Antonio Bayes Genis ,  Manuel Monleon Pradas ,  Carlos Eduardo Semino ,  Philippe Jenny

IPC: A61F2/24 ,  A61L27/26 ,  A61L27/38 ,  A61L27/52 ,  A61L27/54 ,  A61L27/56 ,  A61F2/00 ,  A61L27/58 ,  C12N5/077 ,  C12N5/0775 ,  A61L27/16 ,  A61L27/24

CPC classification number: A61F2/2481 ,  A61F2/0077 ,  A61F2/24 ,  A61F2002/0081 ,  A61F2210/0004 ,  A61F2210/0057 ,  A61F2220/0008 ,  A61F2220/0016 ,  A61F2240/001 ,  A61L27/16 ,  A61L27/24 ,  A61L27/26 ,  A61L27/3834 ,  A61L27/3839 ,  A61L27/3873 ,  A61L27/52 ,  A61L27/54 ,  A61L27/56 ,  A61L27/58 ,  A61L2300/252 ,  A61L2300/44 ,  A61L2300/442 ,  A61L2300/602 ,  A61L2400/12 ,  A61L2430/20 ,  C12N5/0657 ,  C12N5/0663 ,  C12N5/0667 ,  C12N2513/00 ,  C12N2533/30 ,  C08L33/06

Abstract: Bioactive implant for myocardial regeneration and ventricular chamber support including an elastomeric microporous membrane. The elastomeric microporous membrane being at least one non-degradable polymer and at least one partially degradable polymer. The non-degradable polymer is selected from polyethylacrylate and polyethylacrylate copolymerized with a hydroxyethylacrylate comonomer. The partially degradable polymer is selected from caprolactone 2-(methacryloyloxy)ethyl ester and caprolactone 2-(methacryloyloxy)ethyl ester copolymerized with ethylacrylate. The elastomeric microporous membrane further includes a nanofiber hydrogel, and cells. The bioactive implant, having one or two helical loops, contributes to the restoration of the heart conical shape. Cardiac wrapping by ventricular support bioprostheses of the present invention, having reinforcement bands spatially distributed as helicoids, recovers the sequential contraction of the myocardium resulting in the successive shortening and lengthening of the ventricles, therefore improving the ejection (systolic function) and suction of blood (diastolic function).

公开(公告)号：US09986734B2

公开(公告)日：2018-06-05

申请号：US14749889

申请日：2015-06-25

Applicant: YALE UNIVERSITY ,  RAREDON RESOURCES, INC.

Inventor： Micha Sam Brickman Raredon ,  Tom Raredon ,  Laura Niklason

IPC: A01N1/02 ,  A61K35/42 ,  C12M3/00 ,  C12M1/00 ,  C12M1/12 ,  C12M1/34 ,  C12M1/36 ,  A61L27/38 ,  G01N33/50 ,  C12N5/071

CPC classification number: A01N1/0263 ,  A61K35/42 ,  A61L27/3804 ,  A61L27/3839 ,  A61L27/3895 ,  A61L2430/40 ,  C12M21/08 ,  C12M23/00 ,  C12M23/50 ,  C12M23/52 ,  C12M25/14 ,  C12M29/10 ,  C12M41/46 ,  C12M41/48 ,  C12N5/0688 ,  C12N2533/90 ,  G01N33/5088

Abstract: The present invention provides a bioreactor for large-mammal lung tissue. The bioreactor is capable of hydraulic driven negative-pressure and positive-pressure perfusion and ventilation. Perfusion and ventilation is delivered at physiological rates and is easily controllable. In one embodiment, the bioreactor comprises a support scaffold to support the size of a large-mammal lung tissue. In another embodiment, the bioreactor comprises a pleural sack that provides a small isolated fluid chamber which surrounds an engineered lung, thereby minimizing the amount of culture media needed. The present invention also provides an in vitro model for examining the function of a test agent and compositions and methods for alleviating a lung defect in a large-mammal.

公开(公告)号：US20180119103A1

公开(公告)日：2018-05-03

申请号：US15859375

申请日：2017-12-30

Applicant: CELLRESEARCH CORPORATION PTE LTD.

Inventor： Toan-Thang PHAN

IPC: C12N5/077 ,  A61L27/18 ,  A61K35/32 ,  A61L27/36 ,  C12N5/071 ,  C12N5/0775 ,  C12N5/073 ,  A61L27/60 ,  A61L27/56 ,  A61L27/38 ,  C08L69/00

CPC classification number: C12N5/0655 ,  A61K35/32 ,  A61L27/18 ,  A61L27/3633 ,  A61L27/3804 ,  A61L27/3839 ,  A61L27/56 ,  A61L27/60 ,  C12N5/0605 ,  C12N5/0629 ,  C12N5/0668 ,  C12N5/0676 ,  C12N5/0698 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2502/094 ,  C12N2502/1323 ,  C12N2506/02 ,  C12N2506/1369 ,  C12N2533/54 ,  C08L69/00

Abstract: The present invention relates to a method of promoting skin rejuvenation in a subject. The method comprises administering to the subject an effective amount of a cellular extract of epithelial or mesenchymal stem/progenitor cells isolated from the amniotic membrane of the umbilical cord, wherein the cellular extract contains growth factors and peptides and is in the form of a supernatant into which the epithelial or mesenchymal stem/progenitor cells secrete the growth factors and peptides.

公开(公告)号：US09956317B2

公开(公告)日：2018-05-01

申请号：US15131441

申请日：2016-04-18

Applicant: Antria, Inc.

Inventor： Shahram Rahimian

IPC: C12N9/00 ,  C12N5/071 ,  A61L27/38 ,  A61K35/35 ,  A61L27/36 ,  A61L15/44 ,  A61L15/40 ,  A61L27/54 ,  C12N5/0775

CPC classification number: A61L27/3687 ,  A61K35/35 ,  A61L15/40 ,  A61L15/44 ,  A61L27/3604 ,  A61L27/3641 ,  A61L27/3834 ,  A61L27/3839 ,  A61L27/54 ,  A61L2300/412 ,  A61L2300/64 ,  A61L2430/34 ,  C12N5/0667 ,  C12N2500/14 ,  C12N2500/16 ,  C12N2500/22 ,  C12N2509/00

Abstract: Methods and kits for producing cellular fractions enriched in adipose derived stem cells. Methods are provided where adipose tissue obtained from liposuction is enzymatically treated using a solution containing collagenase and divalent cations prior to the application of traditional methods of stromal-vascular fraction isolation. The enzymatic solutions may contain collagenase types I and II to a final concentration of about 0.001 mg/ml to 0.010 mg/ml. The divalent cations may be present as calcium, magnesium, and zinc chloride. The final concentration of calcium, magnesium, and zinc may range from about 0.001 to 0.1 micromolar; about 0.005 to 0.5 micromolar; and about 0.0015 to 0.15 micromolar, respectively. The enzymatic solutions may be generated using a kit where the collagenase and divalent components are held in separate containers until just prior to use. The cellular fractions isolated in this manner may be used in autologous fat grafts in therapeutic applications.