公开(公告)号：US20220283176A1

公开(公告)日：2022-09-08

申请号：US17686224

申请日：2022-03-03

Applicant: Immatics Biotechnologies GmbH

Inventor： Christoph SCHRAEDER ,  Heiko SCHUSTER ,  Lena Katharina FREUDENMANN ,  Lida ROSTOCK ,  Linus BACKERT ,  Michael ROEMER ,  Daniel Johannes KOWALEWSKI

IPC: G01N33/68 ,  G01N1/34

Abstract: The present invention relates to a method for the absolute quantification of one or more MHC molecules in a test sample comprising at least one cell, the method comprising at least the steps of: homogenizing the sample, adding an internal standard to the sample, digesting the homogenized sample with a protease, before or after addition of the internal standard, purifying the sample obtained by the digestion, subjecting the digested sample to a step of chromatography and/or spectrometry analysis, and quantifying the one or more MHC molecules in the test sample Also, the invention relates to method of determining the cell count in a sample. (FIG. 1).