公开(公告)号：US20190247433A1

公开(公告)日：2019-08-15

申请号：US16271393

申请日：2019-02-08

Applicant: Immatics US, Inc.

Inventor： Mamta KALRA ,  Zoe COUGHLIN ,  Amir ALPERT ,  Steffen WALTER ,  Ali MOHAMED ,  Agathe BOURGOGNE

IPC: A61K35/17 ,  C12N5/0783 ,  C07K16/28 ,  C12N15/86 ,  A61P35/00 ,  A61P31/00

CPC classification number: A61K35/17 ,  A61P31/00 ,  A61P35/00 ,  C07K16/2809 ,  C07K16/2818 ,  C12N5/0636 ,  C12N5/0637 ,  C12N5/0638 ,  C12N15/86 ,  C12N2501/2302 ,  C12N2501/2307 ,  C12N2501/2312 ,  C12N2501/2315 ,  C12N2501/2321 ,  C12N2501/51 ,  C12N2501/515 ,  C12N2510/00

Abstract: The disclosure relates to methods of manufacturing T cells for adoptive immunotherapy. The disclosure further provides for methods of genetically transducing T cells, methods of using T cells, and T cell populations thereof. In an aspect, the disclosure provides for methods of thawing frozen peripheral blood mononuclear cells (PBMC), resting the thawed PBMC, activating the T cell in the cultured PBMC with an anti-CD3 antibody and an anti-CD28 antibody immobilized on a solid phase, transducing the activated T cell with a viral vector, expanding the transduced T cell, and obtaining expanded T cells.