公开(公告)号：US20220246235A1

公开(公告)日：2022-08-04

申请号：US17726250

申请日：2022-04-21

Applicant: Inscripta, Inc.

Inventor： Andrea HALWEG-EDWARDS ,  Joshua SHORENSTEIN ,  Andrew GARST ,  Craig STRUBLE ,  Miles GANDER ,  Juhan KIM ,  Bryan LELAND ,  Eileen SPINDLER ,  Paul HARDENBOL ,  Aaron BROOKS ,  Eric ABBATE

IPC: G16B20/30 ,  G16B40/20 ,  C12N15/10 ,  G06N3/08

Abstract: The present disclosure is drawn to creating cassette designs for nucleic acid-guided nuclease editing. In designing editing cassettes, a set of edit specifications must first be obtained. These edit specifications are taken together with a set of configuration parameters to start a computational pipeline that generates a collection of cassette designs. The process of designing editing cassettes involves the following exemplary steps: 1) creation of a set of candidate cassette designs for each unique edit specification, 2) enumeration of features describing biophysical characteristics of each candidate design, 3) providing each candidate design with a score, and 4) returning a number of scored and rank-ordered candidate cassette designs for each edit specification.

公开(公告)号：US20210317445A1

公开(公告)日：2021-10-14

申请号：US16945575

申请日：2020-07-31

Applicant: Inscripta, Inc.

Inventor： Andrea HALWEG-EDWARDS ,  Joshua SHORENSTEIN ,  Andrew GARST ,  Craig STRUBLE ,  Miles GANDER ,  Juhan KIM ,  Bryan LELAND ,  Eileen SPINDLER ,  Paul HARDENBOL

IPC: C12N15/10

Abstract: The present disclosure is drawn to creating cassette designs for nucleic acid-guided nuclease editing. In designing editing cassettes, a set of edit specifications must first be obtained. These edit specifications are taken together with a set of configuration parameters to start a computational pipeline that generates a collection of cassette designs. The process of designing editing cassettes involves the following exemplary steps: 1) creation of a set of candidate cassette designs for each unique edit specification, 2) enumeration of features describing biophysical characteristics of each candidate design, 3) providing each candidate design with a score, and 4) returning a number of scored and rank-ordered candidate cassette designs for each edit specification.

公开(公告)号：US20210317444A1

公开(公告)日：2021-10-14

申请号：US16903324

申请日：2020-06-16

Applicant: Inscripta, Inc.

Inventor： Andrea HALWEG-EDWARDS ,  Joshua SHORENSTEIN ,  Andrew GARST ,  Craig STRUBLE ,  Miles GANDER ,  Juhan KIM ,  Bryan LELAND ,  Eileen SPINDLER ,  Paul HARDENBOL

IPC: C12N15/10

Abstract: The present disclosure is drawn to creating cassette designs for nucleic acid-guided nuclease editing. In designing editing cassettes, a set of edit specifications must first be obtained. These edit specifications are taken together with a set of configuration parameters to start a computational pipeline that generates a collection of cassette designs. The process of designing editing cassettes involves the following exemplary steps: 1) creation of a set of candidate cassette designs for each unique edit specification, 2) enumeration of features describing biophysical characteristics of each candidate design, 3) providing each candidate design with a score, and 4) returning a number of scored and rank-ordered candidate cassette designs for each edit specification.