Methods and compositions for processes of rapid selection and production of nucleic acid aptamers
    1.
    发明授权
    Methods and compositions for processes of rapid selection and production of nucleic acid aptamers 有权
    快速选择和生产核酸适体的方法和组合物

    公开(公告)号:US09273345B2

    公开(公告)日:2016-03-01

    申请号:US11965039

    申请日:2007-12-27

    IPC分类号: C12N15/115 C12Q1/68

    摘要: Embodiments herein relate to compositions and methods for making and using aptamers, for example, DNA aptamers (DCEs) and/or RNA aptamers. In some embodiments, methods relate to making and amplifying target DCEs. In certain embodiments, methods for making capture elements or aptamers concern using a reporter moiety and signal reducing moiety prior to amplifying a target-specific capture element. In some embodiments, methods disclosed herein may be used to rapidly generate large quantities of aptamers such as DCEs directed to a particular target agent. Some embodiments relate to systems for performing automated generation of aptamers.

    摘要翻译: 本文的实施方案涉及用于制备和使用适体的组合物和方法,例如DNA适体(DCE)和/或RNA适体。 在一些实施方案中,方法涉及制备和扩增目标DCE。 在某些实施方案中,用于制备捕获元件或适体的方法在放大靶特异性捕获元件之前关注使用报道部分和信号降低部分。 在一些实施方案中,本文公开的方法可用于快速产生大量适配体,例如针对特定目标试剂的DCE。 一些实施例涉及用于执行适配体的自动生成的系统。

    METHODS AND COMPOSITIONS FOR PROCESSES OF RAPID SELECTION AND PRODUCTION OF NUCLEIC ACID APTAMERS
    2.
    发明申请
    METHODS AND COMPOSITIONS FOR PROCESSES OF RAPID SELECTION AND PRODUCTION OF NUCLEIC ACID APTAMERS 有权
    快速选择和生产核酸APTAMERS的方法和组合物

    公开(公告)号:US20090004644A1

    公开(公告)日:2009-01-01

    申请号:US11965039

    申请日:2007-12-27

    IPC分类号: C12Q1/70 C12Q1/68 G01N33/566

    摘要: Embodiments herein relate to compositions and methods for making and using aptamers, for example, DNA aptamers (DCEs) and/or RNA aptamers. In some embodiments, methods relate to making and amplifying target DCEs. In certain embodiments, methods for making capture elements or aptamers concern using a reporter moiety and signal reducing moiety prior to amplifying a target-specific capture element. In some embodiments, methods disclosed herein may be used to rapidly generate large quantities of aptamers such as DCEs directed to a particular target agent. Some embodiments relate to systems for performing automated generation of aptamers.

    摘要翻译: 本文的实施方案涉及用于制备和使用适体的组合物和方法,例如DNA适体(DCE)和/或RNA适体。 在一些实施方案中,方法涉及制备和扩增目标DCE。 在某些实施方案中,用于制备捕获元件或适体的方法在放大靶特异性捕获元件之前关注使用报道部分和信号降低部分。 在一些实施方案中,本文公开的方法可用于快速产生大量适配体,例如针对特定目标试剂的DCE。 一些实施例涉及用于执行适配体的自动生成的系统。