公开(公告)号：US20240352513A1

公开(公告)日：2024-10-24

申请号：US18753991

申请日：2024-06-25

申请人： Natera, Inc.

发明人： Joshua BABIARZ ,  Tudor Pompiliu CONSTANTIN ,  Lane A. EUBANK ,  George GEMELOS ,  Matthew Micah HILL ,  Huseyin Eser KIRKIZLAR ,  Matthew RABINOWITZ ,  Onur SAKARYA ,  Styrmir SIGURJONSSON ,  Bernhard ZIMMERMANN

IPC分类号： C12Q1/686 ,  C12Q1/68 ,  C12Q1/6811 ,  C12Q1/6848 ,  C12Q1/6874 ,  C12Q1/6883

CPC分类号： C12Q1/686 ,  C12Q1/68 ,  C12Q1/6811 ,  C12Q1/6848 ,  C12Q1/6874 ,  C12Q1/6883 ,  C12Q2600/156

摘要： The invention provides methods, systems, and computer readable medium for detecting ploidy of chromosome segments or entire chromosomes, for detecting single nucleotide variants and for detecting both ploidy of chromosome segments and single nucleotide variants. In some aspects, the invention provides methods, systems, and computer readable medium for detecting cancer or a chromosomal abnormality in a gestating fetus.

公开(公告)号：US20240336970A1

公开(公告)日：2024-10-10

申请号：US18751153

申请日：2024-06-21

申请人： Natera, Inc.

发明人： Matthew Rabinowitz ,  Matthew Micah Hill ,  Bernhard Zimmermann ,  Johan Baner ,  George Gemelos ,  Milena Banjevic ,  Allison Ryan ,  Styrmir Sigurjonsson ,  Zachary Demko

IPC分类号： C12Q1/6883 ,  C12Q1/6809 ,  C12Q1/6811 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6874

CPC分类号： C12Q1/6883 ,  C12Q1/6809 ,  C12Q1/6811 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2600/156

摘要： The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

公开(公告)号：US20240318230A1

公开(公告)日：2024-09-26

申请号：US18442591

申请日：2024-02-15

申请人： Gen-Probe Incorporated

发明人： Mehrdad MAJLESSI ,  Pamela DOUGLASS ,  Daniel KOLK

IPC分类号： C12Q1/6811 ,  C12Q1/6816 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6876 ,  C12Q1/6888 ,  C12Q1/70

CPC分类号： C12Q1/6811 ,  C12Q1/6816 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6876 ,  C12Q1/6888 ,  C12Q1/701 ,  C12Q2600/118 ,  C12Q2600/166

摘要： Compositions, methods, kits, and uses are provided for detecting or quantifying an Human Parainfluenza virus 1 (HPIV-1), HPIV-2, HPIV-3, and/or HPIV-4 nucleic acid, e.g., using nucleic acid amplification and hybridization assays. In some embodiments, the compositions, methods, kits, and uses target the HN gene of HPIV-1, HPIV-2, and/or HPIV-3 and/or the NP gene of HPIV-4.

公开(公告)号：US20240309456A1

公开(公告)日：2024-09-19

申请号：US18678417

申请日：2024-05-30

申请人： Natera, Inc.

发明人： MATTHEW RABINOWITZ ,  Matthew Micah HILL ,  Bernhard A. ZIMMERMANN ,  Johan BANER ,  George GEMELOS ,  Milena BANJEVIC ,  Allison RYAN ,  Styrmir SIGURJONSSON ,  Zachary DEMKO

IPC分类号： C12Q1/6883 ,  C12Q1/6809 ,  C12Q1/6811 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6874

CPC分类号： C12Q1/6883 ,  C12Q1/6809 ,  C12Q1/6811 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2600/156

摘要： The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

公开(公告)号：US20240287708A1

公开(公告)日：2024-08-29

申请号：US18417216

申请日：2024-01-19

申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY ,  THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS

发明人： Joshua Labaer ,  Kevin Gorman ,  Brian Kay ,  Jie Wang ,  Ji Qiu

IPC分类号： C40B30/04 ,  C12N15/10 ,  C12Q1/6811

CPC分类号： C40B30/04 ,  C12N15/1037 ,  C12N15/1041 ,  C12N15/1062 ,  C12Q1/6811

摘要： Methods for screening of affinity reagents for many target proteins of interest simultaneously. Arrayed targets (e.g., peptide, protein, RNA, cell, etc.) are used in affinity selection experiments to reduce the amount of target needed and to improve the throughput of discovering recombinant affinity reagents to a large collection of targets.

公开(公告)号：US11993813B2

公开(公告)日：2024-05-28

申请号：US16643161

申请日：2018-07-06

申请人： MGI TECH CO., LTD.

发明人： Erkai Liu ,  Wenwei Zhang ,  Ao Chen ,  Chongjun Xu

IPC分类号： C12Q1/68 ,  C07H21/00 ,  C12Q1/6811 ,  C12Q1/6853 ,  C12Q1/6874 ,  C12Q1/6855

CPC分类号： C12Q1/6874 ,  C07H21/00 ,  C12Q1/68 ,  C12Q1/6811 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q1/6874 ,  C12Q2521/307 ,  C12Q2525/197 ,  C12Q2531/137 ,  C12Q2537/157 ,  C12Q1/6874 ,  C12Q2523/107 ,  C12Q2525/197 ,  C12Q2531/137 ,  C12Q2537/157

摘要： A nucleic acid probe and a nucleic acid sequencing method for performing sequencing while ligating nucleic acids. The nucleic acid probe is a DNA sequencing probe, comprising a first moiety, a second moiety, a linker, and a detectable label. A base of the first moiety is A, T, U, C, or G, a base of the second moiety is a random base and/or a universal base, and 3 bases or more are present in the second moiety. The first moiety and the second moiety are ligated via the linker, the connection between the first moiety and the ligation can be cleaved, and the detectable label is ligated to the second moiety or the linker. The above probe, a combination formed therewith, or a sequencing method using the same can reduce the number or types of probes in nucleic acid sequencing, thereby reducing cost.

公开(公告)号：US11981965B2

公开(公告)日：2024-05-14

申请号：US18100133

申请日：2023-01-23

申请人： 10x Genomics, Inc.

发明人： James Michael Chell ,  Marlon Stoeckius ,  Jonathan Alles ,  Caroline Julie Gallant ,  Christina Galonska ,  Felice Alessio Bava ,  Layla Katiraee

IPC分类号： C12Q1/6876 ,  C12Q1/25 ,  C12Q1/6811 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6851

CPC分类号： C12Q1/6876 ,  C12Q1/25 ,  C12Q1/6811 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6851

摘要： Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.

公开(公告)号：US11970787B2

公开(公告)日：2024-04-30

申请号：US17248722

申请日：2021-02-04

申请人： Life Technologies Corporation

发明人： Timothy Looney ,  Geoffrey Lowman ,  Lifeng Lin

IPC分类号： C40B50/06 ,  C12Q1/6811 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q1/6883 ,  C07K14/725

CPC分类号： C40B50/06 ,  C12Q1/6811 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q1/6883 ,  C07K14/7051 ,  C12Q2600/136 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2600/166

摘要： The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, target-specific primer panels provide for the effective amplification of sequences of T cell receptor and/or B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Variable regions associated with the immune cell receptor are resolved to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.

公开(公告)号：US20240124933A1

公开(公告)日：2024-04-18

申请号：US18483280

申请日：2023-10-09

申请人： 10x Genomics, Inc.

发明人： James Michael Chell ,  Marlon Stoeckius ,  Jonathan Alles ,  Caroline Julie Gallant ,  Christina Galonska ,  Felice Alessio Bava ,  Layla Katiraee

IPC分类号： C12Q1/6876 ,  C12Q1/25 ,  C12Q1/6811 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6851

CPC分类号： C12Q1/6876 ,  C12Q1/25 ,  C12Q1/6811 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6851

摘要： Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.

公开(公告)号：US20240052414A1

公开(公告)日：2024-02-15

申请号：US18198457

申请日：2023-05-17

申请人： Government of the United States of America, as represented by the Secretary of Commerce

发明人： Veronika Ann Szalai ,  Jacob Michael Majikes

IPC分类号： C12Q1/6876 ,  C12Q1/6811

CPC分类号： C12Q1/6876 ,  C12Q1/6811

摘要： Embodiments of the present invention relate to methods and composition of a nucleic acid tile with addressable locations for placement of molecular species that can function as sensors, reporters, or enhancers of measurement systems. Embodiments of the present invention may also include a nucleic acid tile with addressable locations for placement of molecular species such that attachment chemistry restricts motion of the probes and permits mixing and matching of probes for multiplexed sensing and detection.