公开(公告)号：US20210115509A1

公开(公告)日：2021-04-22

申请号：US16703619

申请日：2019-12-04

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Jae Chun RYU ,  YOON CHO

IPC: C12Q1/6874 ,  C12N15/10

Abstract: Disclosed are a biomarker for identifying specific exposure to ketones, which are one of environmentally harmful substances that may be exposed in the indoor atmosphere environment, and a method for identifying exposure to ketones using the same. More specifically, disclosed are a biomarker composition and a kit for identifying specific exposure to ketones containing genes, the expression of which is increased or decreased specifically in human bronchial epithelial cell lines by exposure to five kinds of ketones including acetone, butanone, pentanone, hexanone and heptanone, and a method for identifying exposure to ketones using the same. Fast-throughput sequencing using a biomarker including a gene showing a changed expression pattern upon exposure to five types of ketones including acetone, butanone, pentanone, hexanone and heptanone is useful for rapidly and accurately monitoring and determining the contamination level of ketones in the environment.

公开(公告)号：US20220073981A1

公开(公告)日：2022-03-10

申请号：US17466881

申请日：2021-09-03

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Jae Chun RYU ,  Yoon CHO

IPC: C12Q1/6876 ,  C12Q1/6874 ,  C12N15/10

Abstract: The present invention provides a biomarker for identifying the expression of an inflammatory-response-associated gene that specifically causes a change in expression due to exposure to 2-butanone, which is harmful and found in indoor environments, and an identification method using the same, and particularly an inflammatory-response-associated gene, the expression of which is increased or decreased by exposure to 2-butanone in a human bronchial epithelial cell line model (BEAS-2B), and a method of identifying exposure and predicting an inflammatory response using the same. The biomarker of the present invention includes specific genes selected through RNA sequencing, and thus can be useful in monitoring and determining the exposure to 2-butanone in the environment, and can be utilized as a tool predicting the mechanism of inflammatory response and toxicity caused by exposure to 2-butanone.