公开(公告)号：US10648024B2

公开(公告)日：2020-05-12

申请号：US15032541

申请日：2014-10-28

Applicant: Korea Institute of Science and Technology

Inventor： Sang Kyung Kim ,  Nakwon Choi ,  Dong Jin Lee ,  Seungwon Jung

IPC: B01L3/00 ,  C12Q1/686

Abstract: A porous structure according to the present invention has a polymerase chain reaction (PCT) primer inside pores thereof, and hence, even an inner portion thereof can be used unlike general structures of which only surfaces are used for amplification and detection, thereby maximizing reactivity. In addition, the differentiating of the kinds of primers contained in respective structures leads to detection of several kinds of target nucleic acids at the same and real-time analysis thereof at the same time, and thus is useful for multiplex real-time PCR.

公开(公告)号：US12257586B2

公开(公告)日：2025-03-25

申请号：US17055167

申请日：2019-05-14

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang Kyung Kim ,  Bong Kyun Kim ,  Jung Min Kim ,  Seungwon Jung

IPC: C12Q1/68 ,  B01L7/00 ,  C09K5/14 ,  C12Q1/686

Abstract: The present invention relates to a porous particle composite for PCR, wherein the porous particle composite distributes photothermal nano-elements that generate heat by absorbing light in porous particles in which nucleic acid amplification occurs through temperature control so as not to adjust the temperature of the entire sample by using a hot plate or the like but to adjust the temperature inside the particles by irradiating light to the porous particles to allow nucleic acid amplification inside thereof, thereby reducing energy consumption and shortening diagnostic time.

公开(公告)号：US10519490B2

公开(公告)日：2019-12-31

申请号：US15499005

申请日：2017-04-27

Applicant: Korea Institute of Science and Technology

Inventor： Sang Kyung Kim ,  Seungwon Jung

IPC: C12Q1/68 ,  C12Q1/6853 ,  C12Q1/686

Abstract: A porous matrix according to the present disclosure, wherein a nucleic acid primer-carbon material composite in which one or more nucleic acid primer of a forward primer and a reverse primer as a polymerase chain reaction (PCR) primer is bound to a carbon material is included in the pores of the matrix, provides improved amplification efficiency as compared to a matrix wherein the nucleic acid primer is present on the outer surface of the matrix or a porous matrix wherein the nucleic acid primer is directly fixed inside pores. The porous matrix of the present disclosure can effectively detect various kinds of target nucleic acids simultaneously and analyze them in real time by varying the kinds of the nucleic acid primers included in the matrix. Therefore, it is useful in amplifying multiple nucleic acids.