公开(公告)号：US20180353648A1

公开(公告)日：2018-12-13

申请号：US15800436

申请日：2017-11-01

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Young Mee JUNG ,  Soo Hyun KIM ,  Yoo Jin SEO

IPC: A61L27/36 ,  A61L27/52 ,  A61L27/24

CPC classification number: A61L27/3687 ,  A61L27/24 ,  A61L27/26 ,  A61L27/3625 ,  A61L27/3691 ,  A61L27/52 ,  C12Y301/00 ,  C12Y304/23001 ,  C12Y304/23002 ,  C08L89/00

Abstract: A method for preparing a decellularized tissue-based hydrogel with maximized ability of preserving various tissue-derived proteins, growth factors and cytokines by using a supercritical fluid-organic solvent system through (a) a step of decellularizing a biological tissue by bringing the same to contact with a supercritical fluid and an organic solvent at the same time; (b) a step of washing the decellularized tissue; (c) a step of preparing a decellularized tissue solution by mixing the washed decellularized tissue with one selected from an enzyme solution, an acidic solution and a mixture thereof; (d) a step of titrating the decellularized tissue solution to pH 5.5-7.8 by treating with a basic solution; and (e) a step of allowing the titrated decellularized tissue solution to stand at 30-40° C. and utilizing the same as a tissue engineering material with improved angiogenesis and tissue regeneration abilities.