公开(公告)号：US20150353977A1

公开(公告)日：2015-12-10

申请号：US14655158

申请日：2013-12-26

Applicant: KOREA RESEARCH INSTITUTE OF CHEMICAL TECHNOLOGY

Inventor： Ju Hyun YU ,  Chan Duck JUNG ,  Seung Hwan LEE ,  In-Chul KIM ,  Kyung Sik HONG ,  In Yong EOM ,  Jong Geon JEGAL ,  Bong Keun SONG

IPC: C12P19/02 ,  C12P19/14

CPC classification number: C12P19/02 ,  C12N1/20 ,  C12P19/14 ,  C12P2201/00

Abstract: The present invention relates to a method for preparing, from wood-based biomass, a high concentration of fermentable sugar which can be effectively used in culturing various industrial fermented bacteria. According to the method of the present invention, biomass can be extracted by hot water prior to a pre-treatment so as to remove extractible substances such as mineral salts to thus minimize the content of impurities in raw materials for an enzymatic saccharification. The biomass from which substances extractible by hot water are removed is pre-treated in the condition where xylan yield rate is maximized, thus achieving maximum inhibition of the generation of over-decomposed products of sugar. Subsequently, fermentable sugar for culturing various industrial fermented bacteria can be prepared in an inexpensive manner by only concentrating, using a separator membrane, the sugar solution obtained by an enzymatic saccharification of the pre-treated solid content obtained by a solid-liquid separation without washing the solid content with water.

Abstract translation: 本发明涉及一种从木质生物质制备高浓度可发酵糖的方法，可发酵糖可有效用于培养各种工业发酵细菌。 根据本发明的方法，可以在预处理之前用热水提取生物质，以除去无机盐等可萃取物质，从而使用于糖化的原料中的杂质含量最小化。 去除由热水提取的物质的生物质在木聚糖产率最大化的条件下进行预处理，从而达到产生过度分解的糖的最大抑制。 随后，可以以便宜的方式制备用于培养各种工业发酵细菌的可发酵糖，其仅通过使用隔膜将通过酶固化获得的糖溶液浓缩得到的预处理固体含量通过固液分离而不洗涤 固体含量与水。