公开(公告)号：US20140170767A1

公开(公告)日：2014-06-19

申请号：US14093305

申请日：2013-11-29

Applicant: Korea Institute of Science and Technology

Inventor： Kwan Hyi LEE ,  Ho Young PARK ,  Yu Chan KIM ,  Hyun Kwang SEOK ,  Seok LEE

IPC: G01N33/543

CPC classification number: G01N33/54326

Abstract: A method for diagnosing a biomarker using magnetic particles and quantum dots for quantitative analysis and a biomarker diagnosis kit are provided. The method for diagnosing a biomarker includes: ii) providing magnetic particles having surfaces to which a primary antibody capable of collecting a biomarker using a linker is fixed; ii) providing quantum dots having surfaces to which a secondary antibody capable of detecting the biomarker is fixed; iii) sandwich-targeting the biomarker by the magnetic particles and the quantum dots; iv) selectively separating quantum dots sandwich-targeting the biomarker among the quantum dots; and v) quantifying the concentration of the biomarker by measuring absorbance or intensity of fluorescence of separated quantum dots.

Abstract translation: 提供了使用磁性粒子和量子点进行定量分析的生物标志物的诊断方法以及生物标志物诊断试剂盒。 用于诊断生物标志物的方法包括：ii）提供具有表面的磁性颗粒，所述表面能够使用接头固定生物标志物的一级抗体; ii）提供具有能够检测生物标志物的二级抗体固定的表面的量子点; iii）通过磁性颗粒和量子点夹心靶向生物标志物; iv）选择性地分离量子点之间的夹心靶向生物标志物的量子点; 和v）通过测量分离的量子点的荧光的吸光度或强度来量化生物标志物的浓度。