公开(公告)号：US08889647B2

公开(公告)日：2014-11-18

申请号：US13742901

申请日：2013-01-16

Applicant: Korea Institute of Science and Technology

Inventor： Hyung-Jun Ahn ,  Ick-Chan Kwon ,  Kui-Won Choi

IPC: C07H21/04 ,  C07K14/00

CPC classification number: C07K14/001 ,  A61K47/62 ,  C07K14/08 ,  C12N15/111 ,  C12N2310/14 ,  C12N2320/32

Abstract: Recombinant proteins for siRNA delivery and a composition including same. These recombinant proteins include a p19 RNA binding protein and a target oriented peptide and can secure the stability of siRNAs from external attacks such as various degradation enzymes, have selective binding affinity to cancer cells by virtue of target-oriented peptides having various cancer cells as their target, and silence target genes by effectively delivering the siRNAs to cells and biological tissues by the release of the siRNAs to the cytoplasms after the cell penetration thereof. Therefore, they are expected to be effectively employed as siRNA delivery vehicles for siRNA therapeutic agents, cell-based drug screening compositions and research.

Abstract translation: 用于siRNA递送的重组蛋白质和包含其的组合物。 这些重组蛋白包括p19 RNA结合蛋白和靶向肽，并且可以确保siRNA从诸如各种降解酶的外部攻击的稳定性，由于具有各种癌细胞的靶向肽具有与癌细胞的选择性结合亲和力 目标和沉默靶基因，通过在siRNA细胞穿透细胞后将siRNA释放到细胞质中，将siRNA有效递送至细胞和生物组织。 因此，预期它们将有效地用作siRNA治疗剂，基于细胞的药物筛选组合物和研究的siRNA递送载体。