公开(公告)号：US20170045430A1

公开(公告)日：2017-02-16

申请号：US15216938

申请日：2016-07-22

Applicant: Korea Institute of Science and Technology

Inventor： Rhokyun KWAK ,  Ji Yoon KANG ,  Jaesung PARK ,  Siwoo CHO

IPC: G01N1/40 ,  G01N27/447 ,  C12Q1/68

CPC classification number: G01N1/40 ,  C12Q1/6806 ,  G01N2001/4038

Abstract: Provided are an extracellular vesicle collecting apparatus using an electrode and a porous membrane and a method for using the same. The extracellular vesicle collecting apparatus includes i) an upper frame in which a pair of through holes is disposed so that a buffer solution flows therethrough, ii) a buffer tube which is inserted in the through holes and in which the buffer solution flows, iii) a positive electrode disposed on the upper frame, iv) a porous membrane located below the upper frame, v) a spacer which is located below the porous membrane and has a hollow space disposed therein, vi) a plurality of guide tubes through which a blood flows in the hollow space, vii) a lower frame which is coupled to the upper frame to be opposite to the upper frame and receives the porous membrane and the spacer therein, and viii) a negative electrode provided below the lower frame. Extracellular vesicles contained in the blood are collected by the porous membrane.

Abstract translation: 提供使用电极和多孔膜的细胞外小泡收集装置及其使用方法。 细胞外囊泡收集装置包括：i）上部框架，其中设置一对通孔，使得缓冲溶液流过其中; ii）缓冲管，其插入通孔中，缓冲溶液流动; iii） 设置在上框架上的正电极，iv）位于上框架下方的多孔膜，v）位于多孔膜下方并具有设置在其中的中空空间的间隔件，vi）多个导管，血液 在中空空间中流动，vii）下框架，其联接到上框架以与上框架相对并且在其中容纳多孔膜和间隔件，以及viii）设置在下框架下方的负极。 通过多孔膜收集血液中所含的细胞外囊泡。