公开(公告)号：US11466328B2

公开(公告)日：2022-10-11

申请号：US16335553

申请日：2017-09-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Ann Mongan ,  Alex Atkins

IPC: C12Q1/6886

Abstract: The present invention provides methods and compositions that are useful for assessing gene expression for tumor immune response profile of a sample. In particular, a target-specific primer panel is provided that allows for selective amplification of immune response target sequences in a sample. In one aspect, the invention relates to target-specific primers useful for selective amplification of one or more target sequences associated with immune response. In some aspects, amplified target sequences obtained using the disclosed methods, and compositions can be used in various processes including nucleic acid sequencing and used to detect the presence of genetic variants and/or expression levels of one or more targeted sequences associated with immune response.

公开(公告)号：US20230088159A1

公开(公告)日：2023-03-23

申请号：US17816214

申请日：2022-07-29

Applicant: Life Technologies Corporation

Inventor： Ann Mongan ,  Alex Atkins

IPC: C12Q1/6886

Abstract: The present invention provides methods and compositions that are useful for assessing gene expression for tumor immune response profile of a sample. In particular, a target-specific primer panel is provided that allows for selective amplification of immune response target sequences in a sample. In one aspect, the invention relates to target-specific primers useful for selective amplification of one or more target sequences associated with immune response. In some aspects, amplified target sequences obtained using the disclosed methods, and compositions can be used in various processes including nucleic acid sequencing and used to detect the presence of genetic variants and/or expression levels of one or more targeted sequences associated with immune response.

公开(公告)号：US11124842B2

公开(公告)日：2021-09-21

申请号：US16503262

申请日：2019-07-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kelli Bramlett ,  Dumitru Brinza ,  Richard Chien ,  Dalia Dhingra ,  Jian Gu ,  Ann Mongan

IPC: C12Q1/6886 ,  C12Q1/6874 ,  C12Q1/6827

Abstract: In some embodiments, the disclosure relates generally to methods, as well as related systems, compositions, kits, apparatuses and computer-readable media, comprising a multiplex molecular tagging procedure that employs a plurality of tags that are appended to a plurality of polynucleotides. The tags have characteristics, including a sequence, length and/or detectable moiety, or any other characteristic, that uniquely identifies the polynucleotide molecule to which it is appended, and permits tracking individual tagged molecules in a mixture of tagged molecules. For example, the tag having a unique tag sequence, can uniquely identify an individual polynucleotide to which it is appended, and distinguish the individual polynucleotide from other tagged polynucleotides in a mixture. In some embodiments, the multiplex molecular tagging procedure can be used for generating error-corrected sequencing data and for detecting a target polynucleotide which is present at low abundance in a nucleic acid sample.

公开(公告)号：US11091811B2

公开(公告)日：2021-08-17

申请号：US16503262

申请日：2019-07-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kelli Bramlett ,  Dumitru Brinza ,  Richard Chien ,  Dalia Dhingra ,  Jian Gu ,  Ann Mongan

IPC: C12Q1/6886 ,  C12Q1/6874 ,  C12Q1/6827

Abstract: In some embodiments, the disclosure relates generally to methods, as well as related systems, compositions, kits, apparatuses and computer-readable media, comprising a multiplex molecular tagging procedure that employs a plurality of tags that are appended to a plurality of polynucleotides. The tags have characteristics, including a sequence, length and/or detectable moiety, or any other characteristic, that uniquely identifies the polynucleotide molecule to which it is appended, and permits tracking individual tagged molecules in a mixture of tagged molecules. For example, the tag having a unique tag sequence, can uniquely identify an individual polynucleotide to which it is appended, and distinguish the individual polynucleotide from other tagged polynucleotides in a mixture. In some embodiments, the multiplex molecular tagging procedure can be used for generating error-corrected sequencing data and for detecting a target polynucleotide which is present at low abundance in a nucleic acid sample.

公开(公告)号：US10344336B2

公开(公告)日：2019-07-09

申请号：US15178450

申请日：2016-06-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kelli Bramlett ,  Dumitru Brinza ,  Richard Chien ,  Dalia Dhingra ,  Jian Gu ,  Ann Mongan

IPC: C12Q1/6827 ,  C12Q1/6874 ,  C12Q1/6886 ,  C07H21/02

Abstract: In some embodiments, the disclosure relates generally to methods, as well as related systems, compositions, kits, apparatuses and computer-readable media, comprising a multiplex molecular tagging procedure that employs a plurality of tags that are appended to a plurality of polynucleotides. The tags have characteristics, including a sequence, length and/or detectable moiety, or any other characteristic, that uniquely identifies the polynucleotide molecule to which it is appended, and permits tracking individual tagged molecules in a mixture of tagged molecules. For example, the tag having a unique tag sequence, can uniquely identify an individual polynucleotide to which it is appended, and distinguish the individual polynucleotide from other tagged polynucleotides in a mixture. In some embodiments, the multiplex molecular tagging procedure can be used for generating error-corrected sequencing data and for detecting a target polynucleotide which is present at low abundance in a nucleic acid sample.