公开(公告)号：US20170335377A1

公开(公告)日：2017-11-23

申请号：US15614582

申请日：2017-06-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： ROBERT GREEN ,  DENNIS WANG ,  JULIO MULERO ,  ROBERT LAGACE

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6888 ,  C12Q2537/143 ,  C12Q2600/156

Abstract: Disclosed are primer set compositions, methods and kits for human identification using the highly complex sequence locus, SE33 (ACTBP2) in single and multiplex PCR reactions. Additionally, disclosed are three newly discovered single nucleotide polymorphisms (SNPs) within the SE33 locus that can cause discordance seen as mobility shift or allelic dropout. Also disclosed are kits useful in human identification.

公开(公告)号：US20170081715A1

公开(公告)日：2017-03-23

申请号：US15367241

申请日：2016-12-02

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： DENNIS WANG ,  Lori Hennessy

IPC: C12Q1/68

Abstract: Disclosed is a method of amplifying a nucleic acid sequence, wherein the method comprises subjecting a reaction mixture to at least one amplification cycle, wherein the reaction mixture comprises a double-stranded nucleic acid and at least two primers capable of annealing to complementary strands of the double-stranded nucleic acid and amplifying at least one short tandem repeat (STR) using a Family A DNA polymerase in a Fast PCR protocol having a two-step amplification cycle in 25 seconds or less. Also disclosed are real-time PCR methods using the two-step protocol and kits for STR profiling using the Fast PCR protocol.