公开(公告)号：US20140316716A1

公开(公告)日：2014-10-23

申请号：US14255528

申请日：2014-04-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Ming Jiang ,  Chengyong Yang ,  Jianxin Zhang

IPC: G06F19/22

CPC classification number: G16B30/00

Abstract: A method includes exposing template polynucleotide strands, sequencing primers, and polymerase to flows of nucleotide species; obtaining a series of measured intensity values and randomly selecting a training subset therefrom; generating series of base calls using a base caller and aligning the series of base calls to a reference genome or sequence using an aligner; determining intensity value thresholds and parameters of a linear transformation corresponding to different homopolymer lengths and nucleotide species; generating series of base calls corresponding to the series of measured intensity values using at least some of the parameters of a linear transformation; and recalibrating the series of base calls corresponding to the plurality of series of measured intensity values using at least some of the intensity value thresholds.

Abstract translation: 一种方法包括将模板多核苷酸链，测序引物和聚合酶暴露于核苷酸物种的流动; 获得一系列测量的强度值并随机选择训练子集; 使用基本呼叫者产生一系列基本呼叫，并使用对准器将一系列基本呼叫对准参考基因组或序列; 确定对应于不同均聚物长度和核苷酸种类的线性转化的强度值阈值和参数; 使用线性变换的至少一些参数来生成对应于所述一系列测量的强度值的一系列基本呼叫; 以及使用至少一些所述强度值阈值来重新校准对应于所述多个测量强度值序列的一系列基本呼叫。

公开(公告)号：US20190034584A1

公开(公告)日：2019-01-31

申请号：US16055315

申请日：2018-08-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Ming Jiang ,  Chengyong Yang ,  Jianxin Zhang

IPC: G06F19/22

Abstract: A method includes exposing template polynucleotide strands, sequencing primers, and polymerase to flows of nucleotide species; obtaining a series of measured intensity values and randomly selecting a training subset therefrom; generating series of base calls using a base caller and aligning the series of base calls to a reference genome or sequence using an aligner; determining intensity value thresholds and parameters of a linear transformation corresponding to different homopolymer lengths and nucleotide species; generating series of base calls corresponding to the series of measured intensity values using at least some of the parameters of a linear transformation; and recalibrating the series of base calls corresponding to the plurality of series of measured intensity values using at least some of the intensity value thresholds.