公开(公告)号：US20240101980A1

公开(公告)日：2024-03-28

申请号：US18521466

申请日：2023-11-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Daniel MAZUR ,  Sihong CHEN ,  Peter VANDER HORN ,  Eileen TOZER ,  Guobin LUO ,  Joshua SHIRLEY ,  Kevin HEINEMANN

IPC: C12N9/12 ,  C12Q1/686

CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Y207/07007

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.

公开(公告)号：US20210284976A1

公开(公告)日：2021-09-16

申请号：US17302621

申请日：2021-05-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Daniel MAZUR ,  Peter B. VANDER HORN ,  Eileen TOZER ,  Sihong CHEN ,  Guobin LUO ,  Joshua SHIRLEY ,  Kevin HEINEMANN

IPC: C12N9/12 ,  C12Q1/686

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.

公开(公告)号：US20190270974A1

公开(公告)日：2019-09-05

申请号：US16415063

申请日：2019-05-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Daniel MAZUR ,  Peter VANDER HORN ,  Eileen TOZER ,  Sihong CHEN ,  Guobin LUO ,  Joshua SHIRLEY ,  Kevin HEINEMANN

IPC: C12N9/12 ,  C12Q1/686

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.

公开(公告)号：US20170096648A1

公开(公告)日：2017-04-06

申请号：US15277834

申请日：2016-09-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： DANIEL MAZUR ,  Peter VANDER HORN ,  Eileen TOZER ,  Sihong CHEN ,  Guobin LUO ,  Joshua SHIRLEY ,  Kevin HEINEMANN

IPC: C12N9/12

CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Y207/07007 ,  C12Q2521/101

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.