公开(公告)号：US20210310024A1

公开(公告)日：2021-10-07

申请号：US17046693

申请日：2019-04-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Maya YOVCHEVA ,  Jonathan ZMUDA ,  Sara BARNES ,  Chang CHOI ,  Kenneth THOMPSON

IPC: C12N15/86 ,  C12N5/07 ,  C12N7/00 ,  C12P21/00

Abstract: The present disclosure is related generally to systems and methods for high level expression of recombinant proteins from baculovirus in insect cells. In particular, the methods and systems described herein allow for high levels of baculovirus production in insect cells and/or high levels of protein production in insect cells using a chemically-defined, yeast lysate-free insect cell medium. The disclosure also relates to compositions and kits for culturing, transfecting, and/or producing recombinant protein in insect cells.