公开(公告)号：US11094398B2

公开(公告)日：2021-08-17

申请号：US14879533

申请日：2015-10-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： James Veitch ,  Yiping Zhan

IPC: G16B40/00 ,  G16B30/00 ,  G16B20/00 ,  C12Q1/6869

Abstract: Methods, systems, and computer-readable media are disclosed for calculating corrected amplicon coverages. One method includes: mapping a plurality of reads of a plurality of amplicons based on amplified target regions of a sample suspected of having one or more genetic abnormalities to a reference sequence that includes one or more nucleic acid sequences corresponding to the amplified target regions; calculating amplicon coverages and total reads, wherein amplicon coverages is a number of reads mapped to an amplicon, and total reads is a number of mapped reads; and calculating corrected amplicon coverages based on the calculated amplicon coverages and calculated total reads by applying a batch effect correction.