公开(公告)号：US10544455B2

公开(公告)日：2020-01-28

申请号：US14856220

申请日：2015-09-16

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES GmbH

Inventor： Wolfgang Hinz ,  Peter Vander Horn ,  Earl Hubbell ,  Christian Woehler

IPC: C12P19/34 ,  C12Q1/6869

Abstract: In some embodiments, the disclosure relates generally to methods, as well as compositions, systems, kits and apparatuses, for performing nucleotide incorporation, comprising: (a) providing a surface including one or more reaction sites containing a polymerase and a nucleic acid template that has, or is hybridized to, an extendible end; (b) performing a first nucleotide flow by contacting one or more of the reaction sites with a first solution including one or more types of terminator nucleotide; (c) incorporating at least one type of terminator nucleotide at the extendible end of the nucleic acid template contained within at least one of the reaction sites using the polymerase; and (d) detecting a non-optical signal indicating the nucleotide incorporation using a sensor that is attached or operatively linked to the at least one reaction site.

公开(公告)号：US11676981B2

公开(公告)日：2023-06-13

申请号：US16624767

申请日：2018-06-18

Applicant: LIFE TECHNOLOGIES GmbH

Inventor： Christian Woehler

IPC: H01L27/146 ,  G02B6/10 ,  G02B5/20

CPC classification number: H01L27/14629 ,  G02B5/204 ,  G02B6/10 ,  H01L27/14621 ,  H01L27/14643

Abstract: An image sensor for recording incident radiation may include a first layer for filtering the incident radiation by attenuating incident radiation with a frequency below a cutoff frequency and a second light-sensitive layer for absorbing radiation passing through the first layer. The first layer may precede the second light-sensitive layer in a direction of propagation of the incident radiation and the first layer includes at least one aperture passing through the first layer to the second light-sensitive layer for propagating radiation therethrough. The cross sectional size of the at least one aperture may be configured to provide a cutoff frequency so that incident radiation with a frequency below the cutoff frequency is attenuated inside the at least one aperture and incident radiation with a frequency above the cutoff frequency propagates through the at least one aperture.