公开(公告)号：US20120009603A1

公开(公告)日：2012-01-12

申请号：US13167137

申请日：2011-06-23

申请人： Leopoldo DELLA CIANA ,  Federica RODEGHIERO ,  Rossana PERCIACCANTE

发明人： Leopoldo DELLA CIANA ,  Federica RODEGHIERO ,  Rossana PERCIACCANTE

IPC分类号： G01N21/76

CPC分类号： G01N33/582 ,  C12Q1/28 ,  G01N33/5306

摘要： Method for increasing and regulating the emission of light from a chemiluminescent reaction including luminol, a peroxidase enzyme, an oxidant and an electron mediator (primary enhancer) through the use of an acylation catalyst (secondary enhancer) belonging to the class of N-azoles., i.e., a class of five-membered nitrogen heteroaromatic ring compounds containing at least one other atom of nitrogen. N-azoles, which are especially useful as secondary enhancers are imidazole, 1-methylimidazole, 1,2,3-triazole and 1,2,4-triazole. The invention also describes the use in diagnostic assays of chemiluminescent substrates containing said N-azoles, as secondary enhancers.

摘要翻译： 通过使用属于N-唑类的酰化催化剂（二级增强剂）来增加和调节包括鲁米诺，过氧化物酶，氧化剂和电子介体（初级增强剂）在内的化学发光反应的光的发射的方法。 ，即一类含有至少一个其他氮原子的五元氮杂芳族环化合物。 作为二次增效剂特别有用的N-吡咯是咪唑，1-甲基咪唑，1,2,3-三唑和1,2,4-三唑。 本发明还描述了在含有所述N-唑类的化学发光底物的诊断测定中用作次级增强剂的用途。