公开(公告)号：US12077784B2

公开(公告)日：2024-09-03

申请号：US18168396

申请日：2023-02-13

Applicant: Life Technologies Corporation

Inventor： Xin Yu ,  Xavier de Mollerat du Jeu ,  Chao Yan Lin ,  Jian Liu ,  Jonathan Zmuda

IPC: C12N1/06 ,  C12N1/22 ,  C12N7/00 ,  C12N15/10 ,  C12N15/86

CPC classification number: C12N7/00 ,  C12N1/06 ,  C12N15/86 ,  C12N2750/14041 ,  C12N2750/14152

Abstract: The instant technology relates to a production system to produce AAV vectors in a serum free suspension platform and at high titers. This technology uses reagents comprising media, cells, transfection reagent, AAV enhancer, and a lysis buffer, each of which is designed to provide maximal AAV production from suspension culture of mammalian cells, e.g. HEK293 cells. With this new system we are able to deliver up to about 2×1011 viral genomes per milliliter (vg/mL) of unconcentrated AAV vectors.

公开(公告)号：US20230193214A1

公开(公告)日：2023-06-22

申请号：US18168396

申请日：2023-02-13

Applicant: Life Technologies Corporation

Inventor： Xin YU ,  Xavier de MOLLERAT du JEU ,  Chao Yan Lin ,  Jian Liu ,  Jonathan Zmuda

IPC: C12N7/00 ,  C12N1/06 ,  C12N15/86

CPC classification number: C12N7/00 ,  C12N1/06 ,  C12N15/86 ,  C12N2750/14041 ,  C12N2750/14152

Abstract: The instant technology relates to a production system to produce AAV vectors in a serum free suspension platform and at high titers. This technology uses reagents comprising media, cells, transfection reagent, AAV enhancer, and a lysis buffer, each of which is designed to provide maximal AAV production from suspension culture of mammalian cells, e.g. HEK293 cells. With this new system we are able to deliver up to about 2×1011 viral genomes per milliliter (vg/mL) of unconcentrated AAV vectors.