公开(公告)号：US11104889B2

公开(公告)日：2021-08-31

申请号：US16633942

申请日：2017-08-09

Applicant: MGI Tech Co., Ltd.

Inventor： Zhougang Zhang ,  Huanhuan Liu ,  Yue Zheng ,  Yujun Zhou ,  Xing Liu ,  Yuliang Dong ,  Chongjun Xu ,  Wenwei Zhang

IPC: C12N9/12

Abstract: Provided are a group of phi29 DNA polymerase mutants having increased thermal stability and use thereof. The phi29 DNA polymerase mutants are proteins obtained by performing point mutation A and/or point mutation B and/or point mutation C on phi29 DNA polymerase, the point mutation A meaning that an amino acid residue M at position 97 of the phi29 DNA polymerase is mutated to other amino acid residue, the point mutation B meaning that an amino acid residue L at position 123 of the phi29 DNA polymerase is mutated into other amino acid residue, and the point mutation C meaning that an amino acid residue E at position 515 of the phi29 DNA polymerase is mutated to other amino acid residue. The stability of the phi29 DNA polymerase mutants is higher than that of a wild-type phi29 DNA polymerase.

公开(公告)号：US20190330602A1

公开(公告)日：2019-10-31

申请号：US16505121

申请日：2019-07-08

Applicant: MGI TECH CO., LTD.

Inventor： Lin Wang ,  Fen Liu ,  Yuliang Dong ,  Wenwei Zhang ,  Chongjun Xu ,  Drmanac Snezana

IPC: C12N9/12 ,  C12Q1/6869

Abstract: Disclosed in the present disclosure is a recombinant DNA polymerase. The recombinant DNA polymerase is any one selected from: A) a protein, having amino acid modifications at positions 408, 409 and 485, and at least one of amino acid modification(s) at positions 53, 59, 199, 243, 526, 558, 613, 641, 671, 673, 674, 692 and 709 compared to the amino acid sequence of a wild-type KOD DNA polymerase; B) a protein derived from the protein in A), formed by deleting amino acids 1 to 29 from a C-terminus of the protein in A) and keeping the remaining amino acids unchanged; and C) a protein derived from the protein in A) or B), formed by connecting a tag to the N-terminus or C-terminus of the amino acid sequence of the protein in A) or B), wherein the protein in A), B) and C) has DNA polymerase activity.

公开(公告)号：US10883092B2

公开(公告)日：2021-01-05

申请号：US16606148

申请日：2017-04-18

Applicant: MGI TECH CO., LTD.

Inventor： Yue Zheng ,  Zhougang Zhang ,  Yuliang Dong ,  Wenwei Zhang ,  Chongjun Xu ,  Snezana Drmanac

IPC: C12N9/12 ,  C12N15/62

Abstract: Provided are a phi29 DNA polymerase and an encoding gene and an application thereof. The phi29 DNA polymerase is C1) or C2): C1) is a protein with DNA polymerase activity obtained by substituting at least one of the 58th, 61st, 94th, 96th, 119th, and 155th amino acid residues in the amino acid sequence of a wild type phi29 DNA polymerase as shown in SEQ ID NO: 2 in the sequence listing; and C2) is a fusion protein obtained by linking a label to the N-terminus and/or C-terminus of the protein represented by C1). A 3′-5′exonuclease of the phi29 DNA polymerase has activity lower than that of the wild type phi29 DNA polymerase, and can efficiently and continuously synthesize DNA during amplification and sequencing.

公开(公告)号：US20200208126A1

公开(公告)日：2020-07-02

申请号：US16633942

申请日：2017-08-09

Applicant: MGI Tech Co., Ltd.

Inventor： Zhougang Zhang ,  Huanhuan Liu ,  Yue Zheng ,  Yujun Zhou ,  Xing Liu ,  Yuliang Dong ,  Chongjun Xu ,  Wenwei Zhang

IPC: C12N9/12

Abstract: Provided are a group of phi29 DNA polymerase mutants having increased thermal stability and use thereof. The phi29 DNA polymerase mutants are proteins obtained by performing point mutation A and/or point mutation B and/or point mutation C on phi29 DNA polymerase, the point mutation A meaning that an amino acid residue M at position 97 of the phi29 DNA polymerase is mutated to other amino acid residue, the point mutation B meaning that an amino acid residue L at position 123 of the phi29 DNA polymerase is mutated into other amino acid residue, and the point mutation C meaning that an amino acid residue E at position 515 of the phi29 DNA polymerase is mutated to other amino acid residue. The stability of the phi29 DNA polymerase mutants is higher than that of a wild-type phi29 DNA polymerase.

公开(公告)号：US10961519B2

公开(公告)日：2021-03-30

申请号：US16657713

申请日：2019-10-18

Applicant: MGI TECH CO., LTD.

Inventor： Huanhuan Liu ,  Yue Zheng ,  Yujun Zhou ,  Xi Zhang ,  Zhougang Zhang ,  Yuliang Dong ,  Wenwei Zhang ,  Chongjun Xu ,  Snezana Drmanac

IPC: C12N9/12 ,  C12N7/00

Abstract: Provided are a phi29 DNA polymerase mutant with increased thermo stability, a method for preparing the mutant, the use of the mutant, and a method for increasing the stability of the phi29 DNA polymerase.

公开(公告)号：US10883091B2

公开(公告)日：2021-01-05

申请号：US16505121

申请日：2019-07-08

Applicant: MGI TECH CO., LTD.

Inventor： Lin Wang ,  Fen Liu ,  Yuliang Dong ,  Wenwei Zhang ,  Chongjun Xu ,  Snezana Drmanac

IPC: C12N9/12 ,  C12Q1/6869

Abstract: Disclosed in the present disclosure is a recombinant DNA polymerase. The recombinant DNA polymerase is any one selected from: A) a protein, having amino acid modifications at positions 408, 409 and 485, and at least one of amino acid modification(s) at positions 53, 59, 199, 243, 526, 558, 613, 641, 671, 673, 674, 692 and 709 compared to the amino acid sequence of a wild-type KOD DNA polymerase; B) a protein derived from the protein in A), formed by deleting amino acids 1 to 29 from a C-terminus of the protein in A) and keeping the remaining amino acids unchanged; and C) a protein derived from the protein in A) or B), formed by connecting a tag to the N-terminus or C-terminus of the amino acid sequence of the protein in A) or B), wherein the protein in A), B) and C) has DNA polymerase activity.