公开(公告)号：US20190330602A1

公开(公告)日：2019-10-31

申请号：US16505121

申请日：2019-07-08

Applicant: MGI TECH CO., LTD.

Inventor： Lin Wang ,  Fen Liu ,  Yuliang Dong ,  Wenwei Zhang ,  Chongjun Xu ,  Drmanac Snezana

IPC: C12N9/12 ,  C12Q1/6869

Abstract: Disclosed in the present disclosure is a recombinant DNA polymerase. The recombinant DNA polymerase is any one selected from: A) a protein, having amino acid modifications at positions 408, 409 and 485, and at least one of amino acid modification(s) at positions 53, 59, 199, 243, 526, 558, 613, 641, 671, 673, 674, 692 and 709 compared to the amino acid sequence of a wild-type KOD DNA polymerase; B) a protein derived from the protein in A), formed by deleting amino acids 1 to 29 from a C-terminus of the protein in A) and keeping the remaining amino acids unchanged; and C) a protein derived from the protein in A) or B), formed by connecting a tag to the N-terminus or C-terminus of the amino acid sequence of the protein in A) or B), wherein the protein in A), B) and C) has DNA polymerase activity.