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公开(公告)号：US11840703B2
公开(公告)日：2023-12-12
申请号：US16917131
申请日：2020-06-30
申请人： Matrexa LLC
CPC分类号： C12N15/86 , C07K14/4725 , A61K9/0014 , A61K38/00
摘要： The disclosure provides methods and constructs for the stable production and isolation of biologically functional recombinant V3, a splice variant of the extracellular matrix proteoglycan versican, as well as the protein product and reagents for detecting the same. In one embodiment, the disclosure provides expression systems for expressing rV3. In one embodiment, the disclosure provides an expression system that properly N-glycosylates mammalian proteins, such as V3. In another embodiment, rV3 has been isolated and purified. In another embodiment, the disclosure provides a unique expression construct containing a ubiquitous chromatin opening element upstream of a strong promoter which drives expression of recombinant V3. In yet a further embodiment, the disclosure provides an isolated and purified recombinant V3 protein in amounts suitable for testing and use, and in pharmaceutical compositions thereof.
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公开(公告)号：US20210024954A1
公开(公告)日：2021-01-28
申请号：US16917131
申请日：2020-06-30
申请人： Matrexa LLC
摘要： The disclosure provides methods and constructs for the stable production and isolation of biologically functional recombinant V3, a splice variant of the extracellular matrix proteoglycan versican, as well as the protein product and reagents for detecting the same. In one embodiment, the disclosure provides expression systems for expressing rV3. In one embodiment, the disclosure provides an expression system that properly N-glycosylates mammalian proteins, such as V3. In another embodiment, rV3 has been isolated and purified. In another embodiment, the disclosure provides a unique expression construct containing a ubiquitous chromatin opening element upstream of a strong promoter which drives expression of recombinant V3. In yet a further embodiment, the disclosure provides an isolated and purified recombinant V3 protein in amounts suitable for testing and use, and in pharmaceutical compositions thereof.
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