公开(公告)号：US11981712B2

公开(公告)日：2024-05-14

申请号：US17084945

申请日：2020-10-30

申请人： NUTRITION & BIOSCIENCES USA 4, INC.

发明人： Qiong Cheng ,  Robert Dicosimo ,  Jahnavi Chandra Prasad ,  Zhenghong Zhang

IPC分类号： C07K14/47 ,  A23L33/10 ,  A23L33/135 ,  A23L33/21 ,  A61K8/60 ,  A61K8/64 ,  A61K8/73 ,  A61K31/716 ,  A61Q19/00 ,  C08B37/00 ,  C08L5/00 ,  C08L5/02 ,  C12N9/10 ,  C12P19/04 ,  C12P19/18 ,  C11D3/22

CPC分类号： C07K14/4725 ,  A23L33/10 ,  A23L33/135 ,  A23L33/21 ,  A61K8/60 ,  A61K8/64 ,  A61K8/73 ,  A61K31/716 ,  A61Q19/00 ,  C08B37/0009 ,  C08L5/00 ,  C08L5/02 ,  C12N9/1051 ,  C12P19/04 ,  C12P19/18 ,  C12Y204/00 ,  C12Y204/01 ,  C12Y204/01005 ,  C12Y302/01 ,  A23V2002/00 ,  A23V2200/00 ,  A61K2800/10 ,  C11D3/22 ,  A23V2002/00 ,  A23V2200/00 ,  A23V2200/32 ,  A23V2200/3202 ,  A23V2200/328 ,  A23V2250/00 ,  A23V2250/282 ,  A23V2250/284 ,  A23V2250/5116

摘要： Disclosed herein are proteins capable of forming glucans having alpha-1,2 linkages/branches, reactions and methods for producing such glucan, compositions comprising such glucan, and various applications thereof.

公开(公告)号：US20240123097A1

公开(公告)日：2024-04-18

申请号：US18473324

申请日：2023-09-25

申请人： Primo Biotechnology Co., Ltd

发明人： Ya-Yao Huang

IPC分类号： A61K51/08 ,  A61K38/17 ,  A61K38/48 ,  C07K14/47 ,  C07K14/705 ,  C12N9/48

CPC分类号： A61K51/08 ,  A61K38/1709 ,  A61K38/177 ,  A61K38/4813 ,  C07K14/4725 ,  C07K14/705 ,  C12N9/485 ,  C12Y304/14005

摘要： A pharmaceutical composition for Positron Emission Tomography (PET) and Boron Neutron Capture Therapy (BNCT) and the use for treating cancer thereof are provided, wherein the pharmaceutical composition comprises a compound having structure of formula I, wherein R1 is a specific peptide, one of R2, R3, and R4 is Fluorine-18 (18F) or Fluorine-19 (19F), one of the other two position is —B(OH)2, and the remaining position is hydrogen (H); a method for preparing a compound having structure of formula I is also provided in the present invention.

公开(公告)号：US11840703B2

公开(公告)日：2023-12-12

申请号：US16917131

申请日：2020-06-30

申请人： Matrexa LLC

发明人： Thomas N. Wight ,  Mervyn J. Merrilees ,  Gernot Kaber ,  Ingrid A. Harten ,  Michael G. Kinsella

IPC分类号： C12P21/06 ,  C12N15/86 ,  C07K14/47 ,  A61K9/00 ,  A61K38/00

CPC分类号： C12N15/86 ,  C07K14/4725 ,  A61K9/0014 ,  A61K38/00

摘要： The disclosure provides methods and constructs for the stable production and isolation of biologically functional recombinant V3, a splice variant of the extracellular matrix proteoglycan versican, as well as the protein product and reagents for detecting the same. In one embodiment, the disclosure provides expression systems for expressing rV3. In one embodiment, the disclosure provides an expression system that properly N-glycosylates mammalian proteins, such as V3. In another embodiment, rV3 has been isolated and purified. In another embodiment, the disclosure provides a unique expression construct containing a ubiquitous chromatin opening element upstream of a strong promoter which drives expression of recombinant V3. In yet a further embodiment, the disclosure provides an isolated and purified recombinant V3 protein in amounts suitable for testing and use, and in pharmaceutical compositions thereof.

公开(公告)号：US11643450B2

公开(公告)日：2023-05-09

申请号：US17097534

申请日：2020-11-13

申请人： UNIVERSITY OF MARYLAND, BALTIMORE

发明人： Lai-Xi Wang ,  Wei Huang

IPC分类号： A61P35/00 ,  C07K14/47 ,  A61K47/68 ,  C12N1/20 ,  C07K16/28 ,  C07K16/00 ,  C07K16/18 ,  C12N9/24 ,  C12P21/00 ,  A61P31/18 ,  C12R1/46

CPC分类号： C07K14/473 ,  A61K47/6849 ,  A61K47/6867 ,  A61P31/18 ,  A61P35/00 ,  C07K14/4725 ,  C07K16/00 ,  C07K16/18 ,  C07K16/2809 ,  C07K16/2887 ,  C12N1/20 ,  C12N1/205 ,  C12N9/2402 ,  C12P21/005 ,  C12Y302/01096 ,  C07K2317/10 ,  C07K2317/14 ,  C07K2317/41 ,  C07K2317/52 ,  C07K2317/72 ,  C07K2317/92 ,  C07K2317/94 ,  C12R2001/46

摘要： The present invention provides for recombinant Endo-S mutants that exhibit reduced hydrolysis activity and increased transglycosylation activity for the synthesis of glycoproteins wherein a desired sialylated oxazoline or synthetic oligosaccharide oxazoline is added to a core fucosylated or nonfucosylated GlcNAc-protein acceptor. Such recombinant Endo-S mutants are useful for efficient glycosylation remodeling of IgG1-Fc domain to provide different antibody glycoforms carrying structurally well-defined Fc N-glycans.

公开(公告)号：US20180282385A1

公开(公告)日：2018-10-04

申请号：US15767689

申请日：2016-11-22

申请人： E I DU PONT DE NEMOURS AND COMPANY

发明人： Qiong Cheng ,  Robert DiCosimo ,  Jahnavi Chandra Prasad ,  Zhenghong Zhang

IPC分类号： C07K14/47 ,  C08B37/00 ,  C08L5/02

CPC分类号： C07K14/4725 ,  A23L33/10 ,  A23L33/135 ,  A23L33/21 ,  A23V2002/00 ,  A23V2200/00 ,  A61K8/60 ,  A61K8/64 ,  A61K8/73 ,  A61K31/716 ,  A61K2800/10 ,  A61Q19/00 ,  C08B37/0009 ,  C08L5/00 ,  C08L5/02 ,  C11D3/22 ,  C12N9/1051 ,  C12P19/04 ,  C12P19/18 ,  C12Y204/00 ,  C12Y204/01 ,  C12Y204/01005 ,  C12Y302/01 ,  A23V2200/32 ,  A23V2200/3202 ,  A23V2200/328 ,  A23V2250/00 ,  A23V2250/282 ,  A23V2250/284 ,  A23V2250/5116

摘要： Disclosed herein are proteins capable of forming glucans having alpha-1,2 linkages/branches, reactions and methods for producing such glucan, compositions comprising such glucan, and various applications thereof.

公开(公告)号：US20170258898A1

公开(公告)日：2017-09-14

申请号：US15454496

申请日：2017-03-09

申请人： Wisconsin Alumni Research Foundation

发明人： Fotis Asimakopoulos ,  Chelsea Hope

IPC分类号： A61K39/39 ,  C07K14/47

CPC分类号： A61K39/39 ,  A61K2039/55516 ,  A61K2039/572 ,  C07K14/4725 ,  C07K2319/00

摘要： Disclosed are methods, kits, polypeptides, and pharmaceutical compositions for inducing an immune response in a subject, which may include a T-cell mediated immune response. The methods comprise administering to the subject, or to explanted cells of the subject, a pharmaceutical composition comprising an effective amount of versikine or a variant of versikine that induces the T-cell mediated immune response. The methods, kits, polypeptides, and pharmaceutical compositions may be used, in particular, to treat a subject having a cell proliferative disease or disorder.

公开(公告)号：US20170058040A1

公开(公告)日：2017-03-02

申请号：US15256854

申请日：2016-09-06

申请人： UNIVERSITY OF MARYLAND, BALTIMORE

发明人： LAI-XI WANG ,  WEI HUANG

IPC分类号： C07K16/28 ,  A61K47/48 ,  C12N9/24 ,  C12N1/20 ,  C12P21/00

CPC分类号： C07K14/473 ,  A61K47/6849 ,  A61K47/6867 ,  A61P31/18 ,  A61P35/00 ,  C07K14/4725 ,  C07K16/00 ,  C07K16/18 ,  C07K16/2809 ,  C07K16/2887 ,  C07K2317/10 ,  C07K2317/14 ,  C07K2317/41 ,  C07K2317/52 ,  C07K2317/72 ,  C07K2317/92 ,  C07K2317/94 ,  C12N1/20 ,  C12N9/2402 ,  C12P21/005 ,  C12R1/46 ,  C12Y302/01096

摘要： The present invention provides for recombinant Endo-S mutants that exhibit reduced hydrolysis activity and increased transglycosylation activity for the synthesis of glycoproteins wherein a desired sialylated oxazoline or synthetic oligosaccharide oxazoline is added to a core fucosylated or nonfucosylated GlcNAc-protein acceptor. Such recombinant Endo-S mutants are useful for efficient glycosylation remodeling of IgG1-Fc domain to provide different antibody glycoforms carrying structurally well-defined Fc N-glycans.

摘要翻译： 本发明提供了表达降低的水解活性和增加糖蛋白合成的转糖基活性的重组Endo-S突变体，其中将所需的唾液酸化恶唑啉或合成寡糖恶唑啉加入核心岩藻糖基化或非岩藻糖基化GlcNAc-蛋白受体中。 这样的重组Endo-S突变体可用于IgG1-Fc结构域的有效糖基化重塑以提供携带结构明确的FcN-聚糖的不同抗体糖基。

公开(公告)号：US09526705B2

公开(公告)日：2016-12-27

申请号：US14990406

申请日：2016-01-07

申请人： Tel-Aviv University Future Technology Development L.P.

发明人： Rimona Margalit ,  Dan Peer

IPC分类号： A61K9/14 ,  A61K9/50 ,  A61K9/00 ,  A61K9/16 ,  A61K9/51 ,  A61K47/48 ,  C07K14/47 ,  A61K47/36 ,  A61K31/165 ,  A61K31/407 ,  A61K31/704 ,  A61K38/21 ,  A61K38/28 ,  A61K38/38 ,  A61K48/00 ,  A61K49/00 ,  A61K51/12 ,  A61K38/00

CPC分类号： A61K9/5036 ,  A61K9/0043 ,  A61K9/1652 ,  A61K9/5123 ,  A61K9/5161 ,  A61K9/5192 ,  A61K31/165 ,  A61K31/407 ,  A61K31/704 ,  A61K38/00 ,  A61K38/212 ,  A61K38/28 ,  A61K38/385 ,  A61K47/36 ,  A61K47/544 ,  A61K47/61 ,  A61K47/6927 ,  A61K47/6939 ,  A61K48/0008 ,  A61K49/0043 ,  A61K49/0091 ,  A61K51/1251 ,  C07K14/4725

摘要： Lipidated glycosaminoglycan particles, prepared by reacting a glycosaminoglycan with at least one lipid to cross-link the carboxylic acid groups in the glycosaminoglycan with a primary amine in the lipid, are used to encapsulate drugs for use in the treatment of pathological conditions in an animal.

摘要翻译： 通过使糖胺聚糖与至少一种脂质反应制备的脂质化糖胺聚糖颗粒用于包封药物用于治疗动物的病理状况，所述糖胺聚糖与糖胺聚糖中的羧酸基团与脂质中的伯胺交联。

公开(公告)号：US20160304572A1

公开(公告)日：2016-10-20

申请号：US15030825

申请日：2014-10-22

申请人： LUBRIS LLC

发明人： Tannin A. Schmidt ,  Gregory D. Jay

IPC分类号： C07K14/47 ,  A61K47/36 ,  A61K38/17 ,  C12P21/00 ,  A61K9/00

CPC分类号： C07K14/4725 ,  A61K9/0014 ,  A61K9/0019 ,  A61K9/0048 ,  A61K9/008 ,  A61K31/728 ,  A61K38/1709 ,  A61K47/36 ,  C12P21/005

摘要： Disclosed are new recombinant isoforms of human-like lubricin or PRG4 glycoprotein having outstanding lubrication properties and a novel glycosylation pattern, and methods for their manufacture at high levels enabling commercial production.

摘要翻译： 公开了具有突出的润滑性能和新型糖基化模式的人样润滑剂或PRG4糖蛋白的新的重组同种型，以及用于在高水平上制造以实现商业生产的方法。

公开(公告)号：US20160222430A1

公开(公告)日：2016-08-04

申请号：US15021599

申请日：2014-09-11

申请人： INDIANA UNIVERSITY RESEARCH AND TECHNOLOGY CORPORATION

发明人： Michael S. Van Nieuwenhze ,  Yves V. Brun ,  Erkin Kuru

IPC分类号： C12Q1/02 ,  C07K5/083 ,  C07K5/103 ,  G01N33/58 ,  C12Q1/10 ,  C12Q1/14 ,  C12Q1/04 ,  C07K5/062 ,  C07K7/06

CPC分类号： C12Q1/02 ,  C07K5/06026 ,  C07K5/0806 ,  C07K5/1008 ,  C07K7/06 ,  C07K14/4725 ,  C12Q1/025 ,  C12Q1/04 ,  C12Q1/10 ,  C12Q1/14 ,  G01N33/5035 ,  G01N33/56911 ,  G01N33/582 ,  G01N2333/245 ,  G01N2333/3156 ,  G01N2333/32 ,  G01N2333/4722 ,  G01N2500/10

摘要： Disclosed herein are compositions for assessing peptidoglycan biosynthesis in bacteria using modified dipeptides containing a bioorthogonal tag and applying novel post-labeling methods to label the bioorthogonal tag. The resultant, labeled peptidoglycan structures are amenable for identifying bacteria by microscopic visualization.

摘要翻译： 本文公开了用于使用包含生物正交标记的修饰的二肽评估细菌中肽聚糖生物合成的组合物，并应用新的后标记方法来标记生物正交标记。 所得到的标记的肽聚糖结构可用于通过显微镜可视化鉴定细菌。