公开(公告)号：US20050283857A1

公开(公告)日：2005-12-22

申请号：US11040472

申请日：2005-01-21

申请人： Michael Adang ,  Gang Hua ,  Jiang Chen ,  Mohd Amir Abdullah

发明人： Michael Adang ,  Gang Hua ,  Jiang Chen ,  Mohd Amir Abdullah

IPC分类号： A01N37/18 ,  A01N63/02 ,  C07K14/435 ,  C07K14/705 ,  C12N15/82

CPC分类号： C12N15/8286 ,  A01N63/02 ,  C07K14/705 ,  Y02A40/162 ,  A01N2300/00

摘要： The subject invention pertains to the use of peptide fragments of cadherins (including cadherin-like proteins). The subject invention includes a cell (and use thereof) comprising a polynucleotide that expresses the peptide fragment. The subject invention includes methods of feeding the peptides to insects. In preferred embodiments, the peptides are fed to target insects together with one or more insecticidal proteins, preferably (but not limited to) B.t. Cry proteins. When used in this manner, the peptide fragment can not only enhance the apparent toxin activity of the Cry protein against the insect species that was the source of the receptor but also against other insect species. Preferably, the cadherin is a Bacillus thuringiensis (B.t.) insecticidal crystal protein (Cry) toxin receptor. Preferably, the peptide fragment is a binding domain of the receptor. In some preferred embodiments, the peptide is the binding domain nearest to the membrane proximal ectodomain. Corresponding domains are identifiable in a variety of B.t. toxin receptors.

摘要翻译： 本发明涉及钙粘蛋白（包括钙粘蛋白样蛋白）的肽片段的用途。 本发明包括含有表达肽片段的多核苷酸的细胞（及其用途）。 本发明包括将肽喂入昆虫的方法。 在优选的实施方案中，将肽与一种或多种杀虫蛋白一起进食至靶昆虫，优选（但不限于）B.t. 哭泣蛋白质 当以这种方式使用时，肽片段不仅可以增强Cry蛋白对作为受体来源的昆虫物种的表观毒素活性，而且还可以抵抗其他昆虫物种。 优选地，钙粘蛋白是苏云金芽孢杆菌（B.t.）杀虫晶体蛋白（Cry）毒素受体。 优选地，肽片段是受体的结合结构域。 在一些优选的实施方案中，肽是最接近膜近端胞外域的结合结构域。 相应的域可以在各种B.t. 毒素受体。

公开(公告)号：US07393922B2

公开(公告)日：2008-07-01

申请号：US10929754

申请日：2004-08-30

申请人： Donald H. Dean ,  Mohd Amir Abdullah

发明人： Donald H. Dean ,  Mohd Amir Abdullah

IPC分类号： C07K5/00 ,  C07H21/02 ,  C12H15/63 ,  C12N1/12

CPC分类号： C07K14/325

摘要： The present invention relates generally to modified Bacillus thuringiensis (Bt) insecticidal crystal proteins, also referred to as mutant toxins, with enhanced toxicity against a variety of insect genera, particularly mosquitos. The invention provides modified Bt Cry4Ba proteins, or mutant toxins, which have toxicity-enhancing sequence modifications at one or more positions within the amino acid sequence of the protein. The invention also provides polynucleotides encoding modified Cry4Ba proteins. The invention also provides insecticidal compositions comprising mutant toxins with a new or broadened insecticidal spectrum, and insecticidal compositions comprising polynucleotides encoding the modified Cry4Ba proteins.

摘要翻译： 本发明一般涉及改良的苏云金芽孢杆菌（Bt）杀虫结晶蛋白，也称为突变毒素，对各种昆虫属，特别是蚊子具有增强的毒性。 本发明提供了修饰的Bt Cry4Ba蛋白或突变毒素，其在蛋白质的氨基酸序列内的一个或多个位置具有毒性增强的序列修饰。 本发明还提供编码修饰的Cry4Ba蛋白的多核苷酸。 本发明还提供包含具有新的或扩大的杀虫谱的突变毒素和包含编码修饰的Cry4Ba蛋白的多核苷酸的杀虫组合物的杀虫组合物。