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公开(公告)号：US20210325654A1
公开(公告)日：2021-10-21
申请号：US17294322
申请日：2019-07-05
Applicant: Nanjing University of Science and Technology
Inventor： Qian CHEN , Chao ZUO , Yao FAN , Jiasong SUN , Jiaji LI , Shijie FENG , Yuzhen ZHANG
Abstract: The patent discloses a differential phase contrast (DPC) quantitative phase microscopy method based on the optimal illumination pattern design. Firstly, the optimal illumination pattern corresponding to the isotropic phase transfer function of DPC quantitative phase imaging is derived, which is determined as a semi-annular illumination pattern with the illumination numerical aperture NAill equal to the numerical aperture NAobj of the objective lens. The illumination intensity distribution varies with the cosine of the illumination angle, and it can be expressed as S(θ)=cos(θ). This patent effectively compensates for the frequency loss of phase transfer, not only the high-frequency responses of PTF are enhanced, but also the transfer responses of low-frequency phase information is significantly improved. As a result, the optimal illumination scheme ensures the correctness and achieves high resolution phase reconstruction, while the number of illuminations is reduced to a minimum of two, which greatly increases the imaging speed, allowing for real-time dynamic, high-correctness, high-resolution phase imaging results.
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公开(公告)号：US20220366552A1
公开(公告)日：2022-11-17
申请号：US17766088
申请日：2020-08-18
Applicant: NANJING UNIVERSITY OF SCIENCE AND TECHNOLOGY
Inventor： Qian CHEN , Yao FAN , Chao ZUO , Jiasong SUN , Xiangpeng PAN , Shijie FENG , Yuzhen ZHANG , Guohua GU , Jiaji LI , Jialin ZHANG
Abstract: A single-shot differential phase contrast quantitative phase imaging method based on color multiplexing illumination. A color multiplexing illumination solution is used to realize single-shot differential phase contrast quantitative phase imaging. In the single-shot color multiplexing illumination solution, three illumination wavelengths of red, green, and blue are used to simultaneously illuminate a sample, and the information of the sample in multiple directions is converted into intensity information on different channels of a color image. By performing channel separation on this color image, the information about the sample at different spatial frequencies can be obtained. Such a color multiplexing illumination solution requires only one acquired image, thus enhancing the transfer response of the phase transfer function of single-shot differential phase contrast imaging in the entire frequency range, and achieving real-time dynamic quantitative phase imaging with a high contrast, a high resolution, and a high stability. In addition, an alternate illumination strategy is provided, so that a completely isotropic imaging resolution at the limit acquisition speed of the camera can be achieved.
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