公开(公告)号：US20220307063A1

公开(公告)日：2022-09-29

申请号：US17615853

申请日：2020-06-24

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Shunsuke TOMITA ,  Ryoji KURITA ,  Hideyuki TAMAKI ,  Hiroyuki KUSADA ,  Naoshi KOJIMA ,  Sayaka ISHIHARA ,  Koyomi MIYAZAKI ,  Isao YUMOTO

IPC: C12Q1/04 ,  G01N21/64

Abstract: A method for analyzing a microbiota, comprising (1) dissolving a probe capable of non-specifically interacting with a plurality of microorganisms in a plurality of solvents having different ionic strengths and pH levels, wherein the probe comprises: (a) a cationic polymer and (b) an environment-sensitive fluorophore; (2) adding a test sample containing the microbiota to a plurality of probe solutions prepared in the step (1), thereby microorganisms in the test sample and the probe are interacted non-specifically; (3) measuring fluorescence intensities of the plurality of probe solutions to which the test sample has been added in the step (2); and (4) comparing the pattern of fluorescence intensities obtained in the step (3) with the pattern of fluorescence intensities obtained from a reference sample.