公开(公告)号：US20130122548A1

公开(公告)日：2013-05-16

申请号：US13747032

申请日：2013-01-22

Applicant: Novozymes A/S

Inventor： Steen Troels Joergensen ,  Jens Toenne Andersen ,  Niels Banke ,  Preben Nielsen

IPC: C12N9/54 ,  C12N9/26

CPC classification number: C12N9/1247 ,  C12N9/2408 ,  C12N9/2417 ,  C12N9/54

Abstract: The present invention relates to an isolated mutant eubacterium comprising at least one mutation resulting in a substitution of at least one amino acid in the beta-subunit of the RNA-polymerase encoded for by the rpoB-gene providing an altered production of a product of interest when said production of a product of interest is compared to the production of the same product in an isogenic wild type strain grown at identical conditions, wherein the substitution of at least one amino acid occurs at any of positions 469, 478, 482, 485, or 487 of SEQ ID NO:2, or at the equivalent positions in any eubacterial RNA-polymererase beta-subunit family member. Another aspect of the invention relates to a process for producing at least one product of interest in a mutant eubacterium and to a use of the mutant eubacterium according to the invention for producing at least one product of interest.

公开(公告)号：US20160115463A1

公开(公告)日：2016-04-28

申请号：US14898502

申请日：2014-06-17

Applicant: Novozymes A/S

Inventor： Jens Toenne Andersen ,  Niels Banke

IPC: C12N9/28

CPC classification number: C12N9/2417 ,  C12Y302/01001

Abstract: The present invention relates to an isolated synthetic polynucleotide encoding the mature amylase AX856 from Bacillus akibai, using codon modified polynucleotide constructs for the expression of the amylase.

Abstract translation: 本发明涉及编码来自芽孢杆菌akibai的成熟淀粉酶AX856的分离的合成多核苷酸，其使用密码子修饰的多核苷酸构建体来表达淀粉酶。

公开(公告)号：US09732331B2

公开(公告)日：2017-08-15

申请号：US14898502

申请日：2014-06-17

Applicant: Novozymes A/S

Inventor： Jens Toenne Andersen ,  Niels Banke

IPC: C12N15/56 ,  C12N9/28

CPC classification number: C12N9/2417 ,  C12Y302/01001

Abstract: The present invention relates to an isolated synthetic polynucleotide encoding the mature amylase AX856 from Bacillus akibai, using codon modified polynucleotide constructs for the expression of the amylase.

公开(公告)号：US20150147783A1

公开(公告)日：2015-05-28

申请号：US14616950

申请日：2015-02-09

Applicant: Novozymes A/S

Inventor： Steen Troels Joergensen ,  Jens Toenne Andersen ,  Niels Banke ,  Preben Nielsen

IPC: C12N9/12 ,  C12N9/54 ,  C12N9/28

CPC classification number: C12N9/1247 ,  C12N9/2408 ,  C12N9/2417 ,  C12N9/54

Abstract: The present invention relates to an isolated mutant eubacterium comprising at least one mutation resulting in a substitution of at least one amino acid in the beta-subunit of the RNA-polymerase encoded for by the rpoB-gene providing an altered production of a product of interest when said production of a product of interest is compared to the production of the same product in an isogenic wild type strain grown at identical conditions, wherein the substitution of at least one amino acid occurs at any of positions 469, 478, 482, 485, or 487 of SEQ ID NO:2, or at the equivalent positions in any eubacterial RNA-polymerase beta-subunit family member. Another aspect of the invention relates to a process for producing at least one product of interest in a mutant eubacterium and to a use of the mutant eubacterium according to the invention for producing at least one product of interest.

Abstract translation: 本发明涉及分离的突变体真细胞，其包含至少一个导致由rpoB基因编码的RNA聚合酶的β-亚基中的至少一个氨基酸取代的突变体，提供改变产生感兴趣的产物 当所述感兴趣的产物的生产与在相同条件下生长的等基因野生型菌株中相同产物的产生进行比较时，其中至少一个氨基酸的取代发生在位置469,478,482,485， 或487，或在任何真细菌RNA聚合酶β-亚基家族成员中的等同位置。 本发明的另一方面涉及用于产生至少一种在突变体杆菌中感兴趣的产物的方法和本发明的突变型杆菌的用途，用于生产至少一种目标产物。